[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=cv. Biggs M-201; DOI=10.1007/BF00016030; PubMed=2491689 [NCBI, ExPASy, EBI, Israel, Japan] Cheng C., Dewdney J., Nam H., den Boer B.G.W., Goodman H.M., Choi H.K., Kleinhofs A., An G.; "Nucleotide sequence of rice nitrate reductase genes."; Plant Mol. Biol. 13:731-733(1989).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=cv. Nipponbare; The international rice genome sequencing project (IRGSP) consortium; "Oryza sativa nipponbare chromosome 8 genomic DNA sequence."; Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=cv. Nipponbare; DOI=10.1126/science.1081288; PubMed=12869764 [NCBI, ExPASy, EBI, Israel, Japan] The rice full-length cDNA consortium; "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."; Science 301:376-379(2003).

Comments

FUNCTION: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
CATALYTIC ACTIVITY: Nitrite + NAD⁺ + H₂O = nitrate + NADH.
COFACTOR: Binds 1 FAD per subunit.
COFACTOR: Binds 1 heme group per subunit.
COFACTOR: Binds 1 molybdenum-pterin group per subunit.
SUBUNIT: Homodimer.
SIMILARITY: Belongs to the nitrate reductase family.
SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.
SIMILARITY: Contains 1 FAD-binding FR-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X15819; CAA33817.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X15820; CAA33817.2; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AP004585; BAD09558.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AK121810; -; NOT_ANNOTATED_CDS; mRNA.	[EMBL / GenBank / DDBJ]

S07554; S07554.

NP_001062006.1; -.

3D structure databases

HSSP

P17571; 2CND. [HSSP ENTRY / PDB]

SMR

P16081; 658-916.

ModBase

P16081.

Organism-specific databases

Gramene

P16081; -.

Ontologies

GO:0009703;	Molecular function: nitrate reductase (NADH) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001199; Cyt_B5.
IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR005066; MoCF_OxRdtse_dimer.
IPR008335; Mopterin_OxRdtase_euk.
IPR012137; Nitr_rd_NADH.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
IPR000572; OxRdtase_Mopterin-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.120.10; Cyt_B5; 1.
G3DSA:2.60.40.650; MoCF_oxrdtse_dimer; 1.
G3DSA:3.90.420.10; Oxred_molyb_bd; 1.

Pfam

PF00173; Cyt-b5; 1.
PF00970; FAD_binding_6; 1.
PF03404; Mo-co_dimer; 1.
PF00175; NAD_binding_1; 1.
PF00174; Oxidored_molyb; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000233; Nitr_rd_NADH; 1.

PRINTS

PR00406; CYTB5RDTASE.
PR00363; CYTOCHROMEB5.
PR00407; EUMOPTERIN.
PR00371; FPNCR.

ProDom

PD000612; Cyt_B5; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00191; CYTOCHROME_B5_1; 1.
PS50255; CYTOCHROME_B5_2; 1.
PS51384; FAD_FR; 1.
PS00559; MOLYBDOPTERIN_EUK; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P16081.

Genome annotation databases

GeneID

4345795; -.

KEGG

osa:4345795; -.

Other

ProtoNet

P16081.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NAD; Nitrate assimilation; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 916`	`916`	`Nitrate reductase [NADH] 1.`	`PRO_0000166065`
`DOMAIN`	`541 616`	`76`	`Cytochrome b5 heme-binding.`
`DOMAIN`	`656 768`	`113`	`FAD-binding FR-type.`
`METAL`	`192 192`		`Molybdenum-pterin (Potential).`
`METAL`	`246 246`		`Molybdenum-pterin (Potential).`
`METAL`	`576 576`		`Iron (heme axial ligand) (By similarity).`
`METAL`	`599 599`		`Iron (heme axial ligand) (By similarity).`
`DISULFID`	`431 431`		`Interchain (Potential).`
`CONFLICT`	`98 98`		`D -> N (in Ref. 1; CAA33817).`
`CONFLICT`	`106 106`		`I -> T (in Ref. 1; CAA33817).`
`CONFLICT`	`129 129`		`R -> E (in Ref. 1; CAA33817).`
`CONFLICT`	`145 145`		`R -> A (in Ref. 1; CAA33817).`
`CONFLICT`	`297 297`		`R -> G (in Ref. 3).`
`CONFLICT`	`370 370`		`H -> Y (in Ref. 1; CAA33817).`
`CONFLICT`	`433 433`		`C -> R (in Ref. 1; CAA33817).`
`CONFLICT`	`436 436`		`S -> P (in Ref. 1; CAA33817).`
`CONFLICT`	`442 442`		`L -> F (in Ref. 1; CAA33817).`
`CONFLICT`	`452 452`		`V -> A (in Ref. 1; CAA33817).`
`CONFLICT`	`581 581`		`D -> N (in Ref. 3).`

Sequence information

Length: 916 AA [This is the length of the unprocessed precursor]

Molecular weight: 101513 Da [This is the MW of the unprocessed precursor]

CRC64: 844D2A0D537E629D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAASVQPRQF GHLEPGSAPV RGAASSNGAK AYPPANGIPR RADSPVRGCG FPPLVSPPPR 

        70         80         90        100        110        120 
KPPSDGSDDE EEEQEDWREL YGSHLQLEVE PPVRDARDEG TADAWIERNP SLIRLTGKHP 

       130        140        150        160        170        180 
LNCEPPLARL MHHGFITPAA LHYVRNHGAV PRGDWSTWTV DVTGLVKRPM RLTMDELVNG 

       190        200        210        220        230        240 
FPAVEIPVTL VCAGNRRKEQ NMVQQTVGFN WGAAGVSTSV WRGARLRDVL RRCGIMPSKG 

       250        260        270        280        290        300 
GALNVCFEGA EDLPGGGGSK YGTSITRQWA LDPSRDIMLA YMQNGEPLLP DHGFPVRAII 

       310        320        330        340        350        360 
PGCIGGRMVK WVKRIIVTTA ESDNYYHYKD NRVLPSHVDA ELANADAWWY KPEYIINELN 

       370        380        390        400        410        420 
VNSVITTPGH DEILPINGIT TQRGYTMKGY AYSGGGKRIT RVEVTLDGGE TWLVCVLDLP 

       430        440        450        460        470        480 
EKPTKYGKHW CWCFWSVEVE VLDLLGAKEI AVRAWDQSHN TQPEKLIWNL MGMMNNCWFK 

       490        500        510        520        530        540 
VKVNVCRPHK GEIGLVFEHP TQPGNQTGGW MARQKHLETA EAAAPGLKRS TSTPFMNTTD 

       550        560        570        580        590        600 
GKQFTMSEVR KHSSQDSAWI VVHGHVYDCT AFLKDHPGGA DSILINAGTD CTEEFDAIHS 

       610        620        630        640        650        660 
DKAKALLDTY RIGELITTGA GYSSDNSVHG ASNLSQLAPI REAIKAPAPV ALSSPRDKVP 

       670        680        690        700        710        720 
CQLVDKKELS RDVRLFRFAL PSSDQVLGLP VGKHIFVCAS IEGKLCMRAY TPTSMVDEVG 

       730        740        750        760        770        780 
HFDLLIKVYF KNEHPKFPDG GLMTQYLDSL PVGAYIDVKG PLGHVEYTGR GEFVINGKPR 

       790        800        810        820        830        840 
NARRLAMIAG GSGITPMYQV IQSVLRDQPE DTTEMHLVYA NRTEDDILLR DELDRWAAEY 

       850        860        870        880        890        900 
PDRLKVWYVI DQVKRPEEGW KYGVGFVTEE VLREHVPEGG DDTLALACGP PPMIKFAVSP 

       910 
NLEKMKYDMA NSFIVF

P16081 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools