NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=MHOM/BR/77/LTB0016/C1S1;
DOI=10.1093/nar/18.9.2819; PubMed=2339068 [NCBI, ExPASy, EBI, Israel, Japan]
Nelson K., Alonso G., Langer P.J., Beverley S.M.;
"Sequence of the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene of Leishmania amazonensis.";
Nucleic Acids Res. 18:2819-2819(1990).

Comments

CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH.
CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.
PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1 .
MISCELLANEOUS: The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
SIMILARITY: In the N-terminal section; belongs to the dihydrofolate reductase family.
SIMILARITY: In the C-terminal section; belongs to the thymidylate synthase family.
SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X51735; CAA36020.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S15756; RDLNTZ.

3D structure databases

HSSP

P04818; 1HVY. [HSSP ENTRY / PDB]

ModBase

P16126.

Family and domain databases

InterPro

IPR012262; DHFR-TS.
IPR001796; DHFR_reg.
IPR000398; Thymidylat_synth_C.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.572.10; Thymidylat_synth_C; 1.

PANTHER

PTHR11549:SF2; Thymidylat_synth_C; 1.

Pfam

PF00186; DHFR_1; 1.
PF00303; Thymidylat_synt; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000389; DHFR-TS; 1.

PRINTS

PR00070; DHFR.
PR00108; THYMDSNTHASE.

ProDom

PD001180; Thymidylat_synth; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03284; thym_sym; 1.

PROSITE

PS00075; DHFR_1; 1.
PS51330; DHFR_2; 1.
PS00091; THYMIDYLATE_SYNTHASE; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; Multifunctional enzyme; NADP; Nucleotide biosynthesis; One-carbon metabolism; Oxidoreductase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 520`	`520`	`Bifunctional dihydrofolate reductase-thymidylate synthase.`	`PRO_0000186344`
`DOMAIN`	`26 229`	`204`	`DHFR.`
`REGION`	`234 520`	`287`	`Thymidylate synthase.`
`ACT_SITE`	`400 400`		`By similarity.`

Sequence information

Length: 520 AA [This is the length of the unprocessed precursor]

Molecular weight: 58610 Da [This is the MW of the unprocessed precursor]

CRC64: B59CEE0CC3CF7BED [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSRAAAKFKI PMPVTKADFA FPSLRAFSIV VALDKQHGIG DGESIPWRVP EDMAFFKDQT 

        70         80         90        100        110        120 
TLLRNKKPPT DKKRNAVVMG RKTWESVPVK FRPLKGRLNV VLSSKATVEE LLAPLPEEKR 

       130        140        150        160        170        180 
AAAAQDIVVV NGGLAAAVRL LARPPYCSSI ETAYCVGGAQ VYADAMLSPC VEKLQEVYLT 

       190        200        210        220        230        240 
RIHTTAPACT RFFPFPPENA ATAWDLASSQ GRRKSAVDGL EFEICKYVPR NHEERQYLEL 

       250        260        270        280        290        300 
IDRIMKTGIA KEDRTGVGTL SLFGAQMRFS LRDNRLPLLT TKRVFWRGVC EELLWFLRGE 

       310        320        330        340        350        360 
TNAQLLADKD IHIWDGNGSR EFLDSRGLTE NTEMDLGPVY GFQWRHFGAE YRGLEANYDG 

       370        380        390        400        410        420 
EGVDQIKFIV ETIKANPNDR RLLFTAWNPC ALHKMALPPC HLLAQFYVNT EKSELSCMLY 

       430        440        450        460        470        480 
QRSCDMGLGV PFNIASYALL TILIAKATGL RPGELVHTLG DAHVYRSHID ALKAQLERVP 

       490        500        510        520 
HAFPTLVFKE ERQFLEDYEL MDMEVIDYVP HPPIKMEMAV

P16126 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools