[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. PubMed=2771943 [NCBI, ExPASy, EBI, Israel, Japan] Thompson E.M., Nagata S., Tsuji F.I.; "Cloning and expression of cDNA for the luciferase from the marine ostracod Vargula hilgendorfii."; Proc. Natl. Acad. Sci. U.S.A. 86:6567-6571(1989).

Comments

CATALYTIC ACTIVITY: Cypridina luciferin + O₂ = oxidized Cypridina luciferin + CO₂ + light.
PTM: The cysteine residues presumably exist in intramolecular disulfide bridges.
PTM: The N-terminus is blocked.
MISCELLANEOUS: 60 kcal/mol are required for the blue emission of light (460 nm) due to the oxidation of luciferin via a dioxetanone intermediate, in which the excited state oxyluciferin bound to luciferase is the emitter.
SIMILARITY: Contains 2 VWFD domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M25666; AAA30332.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A33723; A33723.

3D structure databases

ModBase

P17554.

Ontologies

GO:0047712;	Molecular function: Cypridina-luciferin 2-monooxygenase activity (inferred from electronic annotation from EC).
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Family and domain databases

InterPro

IPR001846; VWF_D.
Graphical view of domain structure.

Pfam

PF00094; VWD; 2.
Pfam graphical view of domain structure.

SMART

SM00216; VWD; 2.
SMART graphical view of domain structure.

PROSITE

PS51233; VWFD; 2.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Decarboxylase; Direct protein sequencing; Glycoprotein; Luminescence; Lyase; Monooxygenase; Oxidoreductase; Photoprotein; Repeat; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 11`	`11`	`Potential.`
`CHAIN`	`12 555`	`544`	`Luciferin 2-monooxygenase.`	`PRO_0000021626`
`DOMAIN`	`81 297`	`217`	`VWFD 1.`
`DOMAIN`	`320 525`	`206`	`VWFD 2.`
`COMPBIAS`	`39 82`	`44`	`Cys-rich.`
`CARBOHYD`	`186 186`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`408 408`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 555 AA [This is the length of the unprocessed precursor]

Molecular weight: 61627 Da [This is the MW of the unprocessed precursor]

CRC64: 2FB150371A80A0DF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKIIILSVIL AYCVTDNCQD ACPVEAEPPS STPTVPTSCE AKEGECIDTR CATCKRDILS 

        70         80         90        100        110        120 
DGLCENKPGK TCCRMCQYVI ECRVEAAGYF RTFYGKRFNF QEPGKYVLAR GTKGGDWSVT 

       130        140        150        160        170        180 
LTMENLDGQK GAVLTKTTLE VAGDVIDITQ ATADPITVNG GADPVIANPF TIGEVTIAVV 

       190        200        210        220        230        240 
EIPGFNITVI EFFKLIVIDI LGGRSVRIAP DTANKGLISG ICGNLEMNDA DDFTTDADQL 

       250        260        270        280        290        300 
AIQPNINKEF DGCPFYGNPS DIEYCKGLME PYRAVCRNNI NFYYYTLSCA FAYCMGGEER 

       310        320        330        340        350        360 
AKHVLFDYVE TCAAPETRGT CVLSGHTFYD TFDKARYQFQ GPCKEILMAA DCYWNTWDVK 

       370        380        390        400        410        420 
VSHRDVESYT EVEKVTIRKQ STVVDLIVDG KQVKVGGVDV SIPYSSENTS IYWQDGDILT 

       430        440        450        460        470        480 
TAILPEALVV KFNFKQLLVV HIRDPFDGKT CGICGNYNQD STDDFFDAEG ACALTPNPPG 

       490        500        510        520        530        540 
CTEEQKPEAE RLCNNLFDSS IDEKCNVCYK PDRIARCMYE YCLRGQQGFC DHAWEFKKEC 

       550 
YIKHGDTLEV PPECQ

P17554 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools