[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1007/BF00280367; PubMed=2325631 [NCBI, ExPASy, EBI, Israel, Japan] Uffen R.L., Colbeau A., Richaud P., Vignais P.M.; "Cloning and sequencing the genes encoding uptake-hydrogenase subunits of Rhodocyclus gelatinosus."; Mol. Gen. Genet. 221:49-58(1990).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.
SEQUENCE CAUTION:
- Sequence=CAA36754.1; Type=Frameshift; Positions=289, 293;

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X52522; CAA36754.1; ALT_FRAME; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S09250; S09250.

3D structure databases

HSSP

P18187; 1FRF. [HSSP ENTRY / PDB]

ModBase

P17633.

Family and domain databases

InterPro

IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006137; OxRdtase_q6.
IPR006311; Tat.
Graphical view of domain structure.

Pfam

PF08425; NiFe_dehyd_N; 1.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000310; NiFe_hyd_ssu; 1.

PRINTS

PR00614; NIHGNASESMLL.

TIGRFAMs

TIGR00391; hydA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3Fe-4S; 4Fe-4S; Cell membrane; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 42`	`42`	`Tat-type signal (Potential).`
`CHAIN`	`43 361`	`319`	`Uptake hydrogenase small subunit.`	`PRO_0000013436`
`METAL`	`59 59`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`62 62`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`156 156`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`190 190`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`228 228`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`231 231`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`256 256`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`262 262`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`271 271`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`290 290`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`293 293`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`

Sequence information

Length: 361 AA [This is the length of the unprocessed precursor]

Molecular weight: 39369 Da [This is the MW of the unprocessed precursor]

CRC64: 2CA41EA36FC81D54 [This is a checksum on the sequence]

        10         20         30         40         50         60 
METFYEVMRR QGISRRSFLK YCSLTATSLG LAPSFVPQIA HAMETKPRTP VLWLHGLECT 

        70         80         90        100        110        120 
CCSESFIRSA HPLAKDAVLS MISLDYDDTL MAAAGHQAEA ILDEIMAKYK GNYILAVEGN 

       130        140        150        160        170        180 
PPLNEDGMFC IQRQALPREA QAVAADCKAV IAWGSCASWG CVQAAKPNPT QATPIHKVIT 

       190        200        210        220        230        240 
DKPIIKVPGC PPIAEVMTGV ITYMLTFDRI PELDRQGRPK MFYSQRIHDK CYRRPHFDAG 

       250        260        270        280        290        300 
QFVESFDDEN ARKGFCLYKV GCKGPTTYNA CSTVMWNEGT SFPIKAGHGC TGCSEDGFWD 

       310        320        330        340        350        360 
KGSFYDRLTN IHQFGIEASA DKVGGTAAGV VGAAIAAHAA ASVIKRLSHD PDAAARAESR 


S

P17633 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools