NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=LT2;
PubMed=2670946 [NCBI, ExPASy, EBI, Israel, Japan]
Ostrowski J., Wu J.-Y., Rueger D.C., Miller B.E., Siegel L.M., Kredich N.M.;
"Characterization of the cysJIH regions of Salmonella typhimurium and Escherichia coli B. DNA sequences of cysI and cysH and a model for the siroheme-Fe4S4 active center of sulfite reductase hemoprotein based on amino acid homology with spinach nitrite reductase.";
J. Biol. Chem. 264:15726-15737(1989).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=LT2 / SGSC1412 / ATCC 700720;
DOI=10.1038/35101614; PubMed=11677609 [NCBI, ExPASy, EBI, Israel, Japan]
McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E., Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R., Wilson R.K.;
"Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
Nature 413:852-856(2001).

Comments

FUNCTION: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.
CATALYTIC ACTIVITY: H₂S + 3 NADP⁺ + 3 H₂O = sulfite + 3 NADPH.
COFACTOR: Binds 1 siroheme per subunit.
COFACTOR: Binds 1 4Fe-4S cluster per subunit.
SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein.
SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M23007; AAA27047.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE008834; AAL21827.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A34354; A34354.

RefSeq

NP_461868.1; -.

3D structure databases

HSSP

P17846; 7GEP. [HSSP ENTRY / PDB]

SMR

P17845; 81-570.

ModBase

P17845.

Enzyme and pathway databases

BioCyc

STYP99287:STM2947-MON; -.

Organism-specific databases

StyGene

SG10076; cysI.

Ontologies

GO:0004783;	Molecular function: sulfite reductase (NADPH) activity (inferred from electronic annotation from HAMAP).
GO:0000103;	Biological process: sulfate assimilation (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01540; -; 1.
PBIL [Tree]

InterPro

IPR011786; CysI.
IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
Graphical view of domain structure.

Pfam

PF01077; NIR_SIR; 1.
PF03460; NIR_SIR_ferr; 2.
Pfam graphical view of domain structure.

PR00397; SIROHAEM.

TIGR02041; CysI; 1.

PS00365; NIR_SIR; 1.

Genome annotation databases

GeneID

1254470; -.

GenomeReviews

AE006468_GR; STM2947.

KEGG

stm:STM2947; -.

NMPDR

fig|99287.1.peg.2843; -.

Phylogenomic databases

HOGENOM

P17845; -.

Genome annotation databases

CMR

P17845; STM2947.

Other

ProtoNet

P17845.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 570`	`569`	`Sulfite reductase [NADPH] hemoprotein beta-component.`	`PRO_0000199908`
`METAL`	`434 434`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`440 440`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`479 479`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`483 483`		`Iron (siroheme axial ligand) (By similarity).`
`METAL`	`483 483`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 570 AA [This is the length of the unprocessed precursor]

Molecular weight: 64023 Da [This is the MW of the unprocessed precursor]

CRC64: 62A9B26F077443C7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSEKHPGPLV VEGKLSDAER MKLESNYLRG TIAEDLNDGL TGGFKGDNFL LIRFHGMYQQ 

        70         80         90        100        110        120 
DDRDIRAERA AQKLEPRHAM LLRCRLPGGV ITTTQWQAID KFAADNTIYG SIRLTNRQTF 

       130        140        150        160        170        180 
QFHGILKKNV KPVHQMLHSV GLDALATAND MNRNVLCTSN PYESQLHAEA YEWAKKISEH 

       190        200        210        220        230        240 
LLPRTRAYAE IWLDQEKVAT TDEEPILGQT YLPRKFKTTV VIPPQNDIDL HANDMNFVAI 

       250        260        270        280        290        300 
AENGKLVGFN LLVGGGLSIE HGNKKTYART ASEFGYLPLE HTLAVAEAVV TTQRDWGNRT 

       310        320        330        340        350        360 
DRKNAKTKYT LERVGLETFK AEVERRAGIK FEPIRPYEFT GRGDRIGWVK GIDDKWHLTL 

       370        380        390        400        410        420 
FIENGRILDY PGRPLKTGLL EIAKIHQGEF RITANQNLII ASVPESQKAK IETLARDHGL 

       430        440        450        460        470        480 
MNAVKPQREN SMACVSFPTC PLAMAEAERF LPSFTDKVEA ILEKHGIPDE HIVMRVTGCP 

       490        500        510        520        530        540 
NGCGRAMLAE IGLVGKAPGR YNLHLGGNRI GSRIPRMYKE NIAEPDILAS LDELIGRWAK 

       550        560        570 
EREAGEGFGD FTVRAGIIRP VLDPARDFWE

P17845 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools