[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=2215219 [NCBI, ExPASy, EBI, Israel, Japan] Ford C.M., Garg N., Garg R.P., Tibelius K.H., Yates M.G., Arp D.J., Seefeldt L.C.; "The identification, characterization, sequencing and mutagenesis of the genes (hupSL) encoding the small and large subunits of the H2-uptake hydrogenase of Azotobacter chroococcum."; Mol. Microbiol. 4:999-1008(1990).
[2]	SEQUENCE REVISION TO 194 AND 564. Yates M.G.; Submitted (SEP-1990) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 428-601. DOI=10.1016/0022-2836(94)90029-9; PubMed=7966281 [NCBI, ExPASy, EBI, Israel, Japan] Du L., Tibelius K.H., Souza E.M., Garg R.P., Yates M.G.; "Sequences, organization and analysis of the hupZMNOQRTV genes from the Azotobacter chroococcum hydrogenase gene cluster."; J. Mol. Biol. 243:549-557(1994).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X52961; CAA37134.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L25315; AAA64446.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S11777; S11777.
S53655; S53655.

3D structure databases

HSSP

P12944; 2FRV. [HSSP ENTRY / PDB]

ModBase

P18191.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0033748;	Molecular function: hydrogenase (acceptor) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 601`	`601`	`Uptake hydrogenase large subunit.`	`PRO_0000199708`
`METAL`	`74 74`		`Nickel (Potential).`
`METAL`	`77 77`		`Nickel (Potential).`
`METAL`	`580 580`		`Nickel (Potential).`
`METAL`	`583 583`		`Nickel (Potential).`
`CONFLICT`	`444 450`		`GLKQFLP -> ASSSSAL (in Ref. 1; CAA37134).`
`CONFLICT`	`457 457`		`L -> S (in Ref. 1; CAA37134).`
`CONFLICT`	`495 498`		`DPST -> GPEH (in Ref. 1; CAA37134).`
`CONFLICT`	`515 517`		`ALG -> RSA (in Ref. 1; CAA37134).`

Sequence information

Length: 601 AA [This is the length of the unprocessed precursor]

Molecular weight: 66546 Da [This is the MW of the unprocessed precursor]

CRC64: 568E6387C2E342FD [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSLPNASQL DKSGRRIVVD PVTRIEGHMR CEVNVDANNI ITNAVSTGTM WRGLEVILKG 

        70         80         90        100        110        120 
RDPRDAWAFV ERICGVCTGT TRWTSVRAVE DALGIQIPYN AHLIRNLMDK QLQVQDHIVP 

       130        140        150        160        170        180 
FYHLLRLDWV NPVNALKADP KATSALPAAL AAHAKSSPGY FRHVQTRLKK FVESGATACS 

       190        200        210        220        230        240 
PNGYWDNPAY QAPARPDLMA VAHYLEALDV QKDIVEIHTI FGGKNPHPNY MVGGVACAIN 

       250        260        270        280        290        300 
LDDVGAAGGR STCTSLNFVL ERIHEAREFT RNVYLPDVLA VAGIYKDWLY GGGLPGHNLL 

       310        320        330        340        350        360 
SYGTFTKVPG DKSSDLLPAG AIVGGNWDEV LPVDVRVPEE IQEFVSHSWY RYADETKGLH 

       370        380        390        400        410        420 
PWDGVTEPKF ELGPNTKGTR TNIKELDEAH KYSWIKARAW RGHAMEVGPL ARYIIAYRSG 

       430        440        450        460        470        480 
REYVKEQVDR SLAAFNQSTG LNLGLKQFLP STLGRTLARA LECELAVDSM LDDWQALVGN 

       490        500        510        520        530        540 
IKAGDRATAN VEKWDPSTWP KEAKGVGINE APRGALGHWI RLKDGKIENY QAIVPTTWNG 

       550        560        570        580        590        600 
TPRDHLGNIG AYEAALLNTR MERPDEPVEI LRTLHSFDPC LACSTHVMSP DGQELTRVKV 


R

P18191 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools