[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=B10; DOI=10.1093/nar/18.17.5285; PubMed=2402452 [NCBI, ExPASy, EBI, Israel, Japan] Schneider C.G., Schmitt H.J., Schild C., Tichy H.V., Lotz W.; "DNA sequence encoding the two structural genes for the uptake hydrogenase of Rhizobium leguminosarum bv. viciae B10."; Nucleic Acids Res. 18:5285-5285(1990).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=128c53; DOI=10.1007/BF00036924; PubMed=2103457 [NCBI, ExPASy, EBI, Israel, Japan] Hidalgo E., Leyva A., Ruiz-Argueso T.; "Nucleotide sequence of the hydrogenase structural genes from Rhizobium leguminosarum."; Plant Mol. Biol. 15:367-370(1990).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z36981; CAA85431.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X52974; CAA37149.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S11969; S11969.

3D structure databases

HSSP

P21852; 1H2R. [HSSP ENTRY / PDB]

ModBase

P18636.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 596`	`596`	`Uptake hydrogenase large subunit.`	`PRO_0000199715`
`METAL`	`75 75`		`Nickel (Potential).`
`METAL`	`78 78`		`Nickel (Potential).`
`METAL`	`575 575`		`Nickel (Potential).`
`METAL`	`578 578`		`Nickel (Potential).`
`CONFLICT`	`510 510`		`R -> A (in Ref. 2; CAA37149).`

Sequence information

Length: 596 AA [This is the length of the unprocessed precursor]

Molecular weight: 66193 Da [This is the MW of the unprocessed precursor]

CRC64: 474EA9B9FD2CBF68 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTIQTPNGFT LDNSGKRIVV DPVTRIEGHM RVEVNVDENN IIRNAVSTGT MWRGIEVILK 

        70         80         90        100        110        120 
NRDPRDAWAF TERICGVCTG THALTSVRAV ENALGITIPD NANSIRNLMQ LALQVHDHVV 

       130        140        150        160        170        180 
HFYHLHALDW VDVVSALSAD PKATSALAQS ISDWPLSSPG YFKDIQTRLK KFVESGQLGP 

       190        200        210        220        230        240 
FKNGYWGNAS YKLPPEANLM AVAHYLEALD FQKEIVKIHT IFGGKNPHPN WLVGGVPCPI 

       250        260        270        280        290        300 
NVDGTGAVGA INMERLNMVT SIIDQLIEFN DKVYVPDIMA IGSFYKDWLY GGGLSGKNVL 

       310        320        330        340        350        360 
AYGDVPEHAN DYSEASLKLP RGAIINGNLA EVFPVDHADP EQIQEFVTHS WYKYPDESKG 

       370        380        390        400        410        420 
LHPWDGITEP HYELGPNAKG TKTNIEQLDE GAKYSWIKAP RWRGNAMEVG PLARWVIGYA 

       430        440        450        460        470        480 
QNKAEFKDPV DKVLKDLGLP VTALFSTLGR TAARALESQW AGYQMRYFQN KLIANIKAGD 

       490        500        510        520        530        540 
SNTAFVDKWK PETWPKEVKG VGFTEAPRGR LAHWIRIKDG KIDNYQCVVP TTWNGSPRDP 

       550        560        570        580        590 
TGNIGAFEAS LMDTPMSNPT QPLEILRTIH SFDPCLACST HVMSPDGQEM ARVQVR

P18636 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools