[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=2404759 [NCBI, ExPASy, EBI, Israel, Japan] Schulze E., Westphal A.H., Hanemaaijer R., de Kok A.; "The 2-oxoglutarate dehydrogenase complex from Azotobacter vinelandii. 1. Molecular cloning and sequence analysis of the gene encoding the 2-oxoglutarate dehydrogenase component."; Eur. J. Biochem. 187:229-234(1990).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 909-943. PubMed=2404760 [NCBI, ExPASy, EBI, Israel, Japan] Westphal A.H., de Kok A.; "The 2-oxoglutarate dehydrogenase complex from Azotobacter vinelandii. 2. Molecular cloning and sequence analysis of the gene encoding the succinyltransferase component."; Eur. J. Biochem. 187:235-239(1990).

Comments

FUNCTION: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).
CATALYTIC ACTIVITY: 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO₂.
COFACTOR: Thiamine pyrophosphate.
SUBUNIT: Homodimer.
SIMILARITY: Belongs to the alpha-ketoglutarate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X52433; CAA36680.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X52432; CAA36677.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S07776; S07776.

3D structure databases

ModBase

P20707.

Family and domain databases

InterPro

IPR011603; 2oxoglutarate_DHase_E1.
IPR001017; DHase_E1.
IPR005475; Transketo_Cen_R.
Graphical view of domain structure.

PANTHER

PTHR23152; 2oxoglutarate_DH_E1; 1.

Pfam

PF00676; E1_dh; 1.
PF02779; Transket_pyr; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000157; Oxoglu_dh_E1; 1.

TIGRFAMs

TIGR00239; 2oxo_dh_E1; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Glycolysis; Oxidoreductase; Thiamine pyrophosphate.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 943`	`943`		`2-oxoglutarate dehydrogenase E1 component.`	`PRO_0000162186`

Sequence information

Length: 943 AA [This is the length of the unprocessed precursor]

Molecular weight: 105688 Da [This is the MW of the unprocessed precursor]

CRC64: D3F35356D454E2A1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQDSVMQRMW NSAHLSGGNA AYVEELYELY LHDPNAVPEE WRTYFEKLPA EAGTSTDVPH 

        70         80         90        100        110        120 
APVRDQFVLL AKNQRRAQPV ATSSVSTEHE KKQVEVLRLI QAYRTRGHQA SQLDPLGLWQ 

       130        140        150        160        170        180 
RTAPSDLSIT HYGLTNADLD TPFRTGELYI GKEEATLREI LQALQETYCR TIGAEFTHIV 

       190        200        210        220        230        240 
DSEQRNWFAQ RLESVRGRPV YSKEAKSHLL ERLSAAEGLE KYLGTKYPGT KRFGLEGGES 

       250        260        270        280        290        300 
LVPVVDEIIQ RSGSYGTKEV VIGMAHRGRL NLLVNALGKN PRDLFDEFEG KHLVELGSGD 

       310        320        330        340        350        360 
VKYHQGFSSN VMTSGGEVHL AMAFNPSHLE IVSPVVEGSV RARQDRRVDA TGEKVVPISI 

       370        380        390        400        410        420 
HGDSAFAGQG VVMETFQMSQ IRGYKTGGTI HIVVNNQVGF TTSNPVDTRS TEYCTDPAKM 

       430        440        450        460        470        480 
IQAPVLHVNG DDPEAVLFVT QLAVDYRMQF KRDVVIDLVC YRRRGHNEAD EPSGTQPLMY 

       490        500        510        520        530        540 
QKIAKQPTTR ELYADALVKE GSLSQEEVQA KVDEYRTALD NGQHVLKSLV KEPNTELFVD 

       550        560        570        580        590        600 
WTPYLGHAWT ARHDTSFELK TLQELNAKLL QIPEGFVVQR QVAKILEDRG RMGVGAMPIN 

       610        620        630        640        650        660 
WGCAETLAYA TLLKEGHPVR ITGQDVGRGT FSHRHAALHN QKDASRYIPL QNLYEGQPKF 

       670        680        690        700        710        720 
ELYDSFLSEE AVLAFEYGYA TTTPNALVIW EASSGDFANG AQVVIDQFIS SGETKWGALC 

       730        740        750        760        770        780 
GLTMLLPHGY EGQGPEHSSA RLERYLQLCA EQNIQVCVPT TPAQVYHMLR RQVIRPLRKP 

       790        800        810        820        830        840 
LVALTPKSLL RHKSAISTLE DLALGSFHPV LPEVDSLDPK KVERLVLCSG KVYYDLLDKR 

       850        860        870        880        890        900 
HAEGREDIAI VRIEQLYPFP EEELAEVMAP YTNLKHVVWC QEEPMNQGAW YCSQHHMRRV 

       910        920        930        940 
ASAHKKELFL QYAGREASAA PACGYASMHA EQQEKLLQDA FTV

P20707 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools