EC 1.3.1.25
Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase
Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase
2-hydro-1,2-dihydroxybenzoate dehydrogenase
DHB dehydrogenase

Gene name

Name:

xylL

From

Pseudomonas putida

[TaxID: 303]

Encoded on

Plasmid TOL pWW0.

Taxonomy

Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1740120 [NCBI, ExPASy, EBI, Israel, Japan]
Neidle E.L., Hartnett C., Ornston L.N., Bairoch A., Rekik M., Harayama S.;
"Cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily.";
Eur. J. Biochem. 204:113-120(1992).

Comments

FUNCTION: Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol.
CATALYTIC ACTIVITY: (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD⁺ = catechol + CO₂ + NADH.
PATHWAY: Aromatic compound metabolism; benzoic acid degradation via hydroxylation; catechol from benzoic acid: step 2/2 .
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M64747; AAA26050.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S23485; S23485.

RefSeq

NP_542868.1; -.

3D structure databases

HSSP

Q9LBG2; 1IY8. [HSSP ENTRY / PDB]

ModBase

P23102.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-2964; -.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

P23102.

Genome annotation databases

GeneID

1218753; -.

Other

ProtoNet

P23102.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Plasmid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 269`	`269`	`1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase.`	`PRO_0000054814`
`NP_BIND`	`11 35`	`25`	`NAD (By similarity).`
`ACT_SITE`	`153 153`		`Proton acceptor (By similarity).`
`BINDING`	`142 142`		`Substrate (By similarity).`

Sequence information

Length: 269 AA [This is the length of the unprocessed precursor]

Molecular weight: 28883 Da [This is the MW of the unprocessed precursor]

CRC64: 23708B9757C6FC41 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNKRFQGKVA VITGAAQGIG RRVAERMAAE GGRLLLVDRS ELIHELADEL VGVAEVLTLT 

        70         80         90        100        110        120 
ADLEQFAECQ RVMAAALERF GRLDILINNV GGTIWAKPFE HYQEREIEAE VRRSLFPTLW 

       130        140        150        160        170        180 
CCHAALAPMI EQGSGAIVNV SSVATRGIHR VPYGAAKGGV NALTACLAFE TAERGIRVNA 

       190        200        210        220        230        240 
TAPGGTEARH GGFRNSAEPS EQEKVWYQQI VDQSLDSSLM KRYGSIDEQV EAILFLASDA 

       250        260 
ASYITGITLP VAGGDLGCQS CSVMFSVSG

P23102 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools