Proline dehydrogenase
     (EC 1.5.99.8)
     (Proline oxidase)
Delta-1-pyrroline-5-carboxylate dehydrogenase
     (P5C dehydrogenase)
     (EC 1.5.1.12)

Gene name

Name:

putA

From

Klebsiella pneumoniae

[TaxID: 573]

Taxonomy

Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Klebsiella.

Protein existence

2: Evidence at transcript level;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=1987164 [NCBI, ExPASy, EBI, Israel, Japan] Chen L.M., Maloy S.; "Regulation of proline utilization in enteric bacteria: cloning and characterization of the Klebsiella put control region."; J. Bacteriol. 173:783-790(1991).

Comments

FUNCTION: Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.
CATALYTIC ACTIVITY: L-proline + acceptor + H₂O = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
CATALYTIC ACTIVITY: (S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H.
COFACTOR: FAD.
PATHWAY: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2 .
PATHWAY: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2 .
INDUCTION: By proline, autorepression and catabolite repression, and is potentially nitrogen controlled.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M63160; AAA25139.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P23725.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
Graphical view of domain structure.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; PARTIAL.
PS00687; ALDEHYDE_DEHYDR_GLU; PARTIAL.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

DNA-binding; FAD; Flavoprotein; Multifunctional enzyme; NAD; Oxidoreductase; Proline metabolism; Repressor; Transcription; Transcription regulation.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 >26`	`>26`	`Bifunctional protein putA.`	`PRO_0000056526`
`NON_TER`	`26 26`

Sequence information

Length: 26 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 2824 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: BB332D0DE504CE19 [This is a checksum on the sequence]

        10         20 
MGTTTMGVKL DDATRERIKS AASRID

P23725 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools