ID   LUXA_VIBAB              Reviewed;         103 AA.
AC   P24114;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-NOV-2008, entry version 50.
DE   RecName: Full=Alkanal monooxygenase alpha chain;
DE            EC=1.14.14.3;
DE   AltName: Full=Bacterial luciferase alpha chain;
DE   Flags: Fragment;
GN   Name=luxA;
OS   Vibrio albensis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=140100;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 14547 / LMG 4406 / NCIMB 41, UM4082, and UM4102;
RX   MEDLINE=91307297; PubMed=1854193;
RA   Palmer L.M., Colwell R.R.;
RT   "Detection of luciferase gene sequence in nonluminescent Vibrio
RT   cholerae by colony hybridization and polymerase chain reaction.";
RL   Appl. Environ. Microbiol. 57:1286-1293(1991).
CC   -!- FUNCTION: Light-emitting reaction in luminous bacteria.
CC   -!- CATALYTIC ACTIVITY: RCHO + reduced FMN + O(2) = RCOOH + FMN +
CC       H(2)O + light.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC   -!- MISCELLANEOUS: The synthesis of this protein is regulated by a
CC       complex control mechanism that has been termed autoinduction. In
CC       fully induced cells luciferase comprises up to 5% of the soluble
CC       protein.
CC   -!- MISCELLANEOUS: Strain ATCC 14547 is luminescent.
CC   -!- MISCELLANEOUS: Strain UM4102 is dimly luminescent.
CC   -!- MISCELLANEOUS: Strain UM4082 is non-luminescent.
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DR   EMBL; M60437; AAA27532.1; -; Genomic_DNA.
DR   EMBL; M60438; AAA27533.1; -; Genomic_DNA.
DR   EMBL; M60439; AAA27534.1; -; Genomic_DNA.
DR   PIR; B49775; B49775.
DR   PIR; C49775; C49775.
DR   HSSP; P07740; 1BRL.
DR   SMR; P24114; 1-103.
DR   GO; GO:0047646; F:alkanal monooxygenase (FMN-linked) activity; IEA:EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR011251; Luciferase-like_bac.
DR   InterPro; IPR002103; Luciferase_bac.
DR   InterPro; IPR016048; Luciferase_mOase.
DR   Gene3D; G3DSA:3.20.20.30; Luciferase_like; 1.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   PRINTS; PR00089; LUCIFERASE.
DR   PROSITE; PS00494; BACTERIAL_LUCIFERASE; PARTIAL.
PE   4: Predicted;
KW   Flavoprotein; FMN; Luminescence; Monooxygenase; Oxidoreductase;
KW   Photoprotein.
FT   CHAIN        <1   >103       Alkanal monooxygenase alpha chain.
FT                                /FTId=PRO_0000220170.
FT   VARIANT     101    101       Y -> F (in strain: UM4082).
FT   VARIANT     101    101       Y -> S (in strain: UM4102).
FT   NON_TER       1      1
FT   NON_TER     103    103
SQ   SEQUENCE   103 AA;  12337 MW;  D0DE5D3EEAB137D2 CRC64;
     INTHEKKAQL DLYNEIALEH GHDIQNIDHC LSYITSVDHD SQRAKDICRQ FLAHWYDSYV
     NATRIFDDSD QTKGYDFNKG QWRDFVLKGH RDTNRRIDYS YEI
//