ID   AL1A1_MOUSE             Reviewed;         501 AA.
AC   P24549; Q7TQJ0; Q811J0;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 5.
DT   25-NOV-2008, entry version 90.
DE   RecName: Full=Retinal dehydrogenase 1;
DE            Short=RALDH 1;
DE            Short=RalDH1;
DE            EC=1.2.1.36;
DE   AltName: Full=Aldehyde dehydrogenase family 1 member A1;
DE   AltName: Full=Aldehyde dehydrogenase, cytosolic;
DE   AltName: Full=ALHDII;
DE   AltName: Full=ALDH-E1;
GN   Name=Aldh1a1; Synonyms=Ahd-2, Ahd2, Aldh1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=BALB/c, and C57BL/6; TISSUE=Liver;
RX   MEDLINE=91276281; PubMed=2055490; DOI=10.1016/0378-1119(91)90421-7;
RA   Rongnoparut P., Weaver S.;
RT   "Isolation and characterization of a cytosolic aldehyde dehydrogenase-
RT   encoding cDNA from mouse liver.";
RL   Gene 101:261-265(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=129/ReJ, BALB/c, and C57BL/6J; TISSUE=Liver;
RX   MEDLINE=95085815; PubMed=7993664; DOI=10.1006/bmmb.1994.1048;
RA   Bond S.L., Singh S.M.;
RT   "DNA sequence analysis of the cytosolic acetaldehyde dehydrogenase
RT   gene (Ahd-2) in mouse strains with variable ethanol preferences.";
RL   Biochem. Med. Metab. Biol. 52:155-159(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Colon, and Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PROTEIN SEQUENCE OF 23-52; 140-156; 211-230; 309-320; 330-349;
RP   400-410; 421-435 AND 477-490.
RC   TISSUE=Embryonic retina;
RX   PubMed=1935685;
RA   McCaffery P., Tempst P., Lara G., Drager U.C.;
RT   "Aldehyde dehydrogenase is a positional marker in the retina.";
RL   Development 112:693-702(1991).
CC   -!- FUNCTION: In addition to the activity on acetaldehyde and related
CC       substrates, is also involved in the oxidation of aldehydes derived
CC       from biogenic amines such as epinephrine and norepinephrine, as
CC       well as the aldehydes generated via lipid peroxidation. Binds free
CC       retinal and cellular retinol-binding protein-bound retinal. Can
CC       convert/oxidize retinaldehyde to retinoic acid (By similarity).
CC   -!- CATALYTIC ACTIVITY: Retinal + NAD(+) + H(2)O = retinoate + NADH.
CC   -!- PATHWAY: Cofactor metabolism; retinol metabolism.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- TISSUE SPECIFICITY: Expressed in the liver, lung, and testis.
CC       Apparently not expressed at detectable levels in kidney, stomach,
CC       ovary, heart, and brain.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; M74570; AAA37202.1; -; mRNA.
DR   EMBL; M74571; AAA37203.1; -; Genomic_DNA.
DR   EMBL; S75713; AAB32754.2; -; mRNA.
DR   EMBL; S77047; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC044729; AAH44729.1; ALT_INIT; mRNA.
DR   EMBL; BC054386; AAH54386.1; -; mRNA.
DR   PIR; JQ1004; JQ1004.
DR   RefSeq; NP_038495.2; -.
DR   UniGene; Mm.457973; -.
DR   HSSP; P51977; 1BXS.
DR   PhosphoSite; P24549; -.
DR   SWISS-2DPAGE; P24549; -.
DR   REPRODUCTION-2DPAGE; P24549; -.
DR   Ensembl; ENSMUSG00000053279; Mus musculus.
DR   GeneID; 11668; -.
DR   KEGG; mmu:11668; -.
DR   MGI; MGI:1353450; Aldh1a1.
DR   HOGENOM; P24549; -.
DR   HOVERGEN; P24549; -.
DR   NextBio; 279287; -.
DR   ArrayExpress; P24549; -.
DR   CleanEx; MM_ALDH1A1; -.
DR   GermOnline; ENSMUSG00000053279; Mus musculus.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004028; F:3-chloroallyl aldehyde dehydrogenase activity; IDA:MGI.
DR   GO; GO:0001758; F:retinal dehydrogenase activity; IEA:EC.
DR   GO; GO:0048048; P:embryonic eye morphogenesis; IGI:MGI.
DR   GO; GO:0002072; P:optic cup morphogenesis involved in camera-...; IGI:MGI.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0043065; P:positive regulation of apoptosis; IGI:MGI.
DR   GO; GO:0042573; P:retinoic acid metabolic process; IMP:MGI.
DR   GO; GO:0042572; P:retinol metabolic process; IMP:MGI.
DR   InterPro; IPR016160; Ald_DHase_CS.
DR   InterPro; IPR016162; Ald_DHase_N.
DR   InterPro; IPR015590; Aldehyde_DHase.
DR   Gene3D; G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   Pfam; PF00171; Aldedh; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Cytoplasm; Direct protein sequencing; NAD;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    501       Retinal dehydrogenase 1.
FT                                /FTId=PRO_0000056417.
FT   NP_BIND     246    251       NAD (By similarity).
FT   REGION      300    305       Antabuse binding.
FT   ACT_SITE    269    269       Proton acceptor (By similarity).
FT   ACT_SITE    303    303       Nucleophile (By similarity).
FT   SITE        170    170       Transition state stabilizer (By
FT                                similarity).
FT   MOD_RES       2      2       N-acetylserine (By similarity).
FT   CONFLICT      8      8       A -> R (in Ref. 1; AAA37202/AAA37203).
FT   CONFLICT     45     45       T -> S (in Ref. 2; AAB32754).
FT   CONFLICT     51     51       H -> Q (in Ref. 2; AAB32754).
FT   CONFLICT     87     87       R -> C (in Ref. 1; AAA37202).
FT   CONFLICT    140    140       I -> Y (in Ref. 4; AA sequence).
FT   CONFLICT    458    458       M -> I (in Ref. 1; AAA37202).
SQ   SEQUENCE   501 AA;  54468 MW;  0E428151B799BD1D CRC64;
     MSSPAQPAVP APLADLKIQH TKIFINNEWH NSVSGKKFPV LNPATEEVIC HVEEGDKADV
     DKAVKAARQA FQIGSPWRTM DASERGRLLN KLADLMERDR LLLATMEALN GGKVFANAYL
     SDLGGCIKAL KYCAGWADKI HGQTIPSDGD IFTYTRREPI GVCGQIIPWN FPMLMFIWKI
     GPALSCGNTV VVKPAEQTPL TALHLASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD
     KVAFTGSTQV GKLIKEAAGK SNLKRVTLEL GGKSPCIVFA DADLDIAVEF AHHGVFYHQG
     QCCVAASRIF VEESVYDEFV KRSVERAKKY VLGNPLTPGI NQGPQIDKEQ HDKILDLIES
     GKKEGAKLEC GGGRWGNKGF FVQPTVFSNV TDEMRIAKEE IFGPVQQIMK FKSVDDVIKR
     ANNTTYGLAA GLFTKDLDKA ITVSSALQAG VVWVNCYMML SAQCPFGGFK MSGNGRELGE
     HGLYEYTELK TVAMKISQKN S
//