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UniProtKB/Swiss-Prot entry P25340


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ERG4_YEAST
Primary accession number P25340
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on October 1, 1996 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 69)
Name and origin of the protein
Protein name Delta(24(24(1)))-sterol reductase
Synonyms EC 1.3.1.71
Sterol Delta(24(28))-reductase
C-24(28) sterol reductase
Gene name
Name: ERG4
OrderedLocusNames: YGL012W
ORFNames: YGL022
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 46191 / IL125-2B;
PubMed=1882555 [NCBI, ExPASy, EBI, Israel, Japan]
Chen W., Capieaux E., Balzi E., Goffeau A.;
"The YGL022 gene encodes a putative transport protein.";
Yeast 7:305-308(1991).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 46191 / IL125-2B;
DOI=10.1002/yea.320070311; PubMed=1882553 [NCBI, ExPASy, EBI, Israel, Japan]
Chen W., Balzi E., Capieaux E., Choder M., Goffeau A.;
"The DNA sequencing of the 17 kb HindIII fragment spanning the LEU1 and ATE1 loci on chromosome VII from Saccharomyces cerevisiae reveals the PDR6 gene, a new member of the genetic network controlling pleiotropic drug resistance.";
Yeast 7:287-299(1991).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169869 [NCBI, ExPASy, EBI, Israel, Japan]
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
Nature 387:81-84(1997).
[4]
CHARACTERIZATION.
DOI=10.1016/0378-1119(94)90728-5; PubMed=8125337 [NCBI, ExPASy, EBI, Israel, Japan]
Lai M.H., Bard M., Pierson C.A., Alexander J.F., Goebl M., Carter G.T., Kirsch D.R.;
"The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway.";
Gene 140:41-49(1994).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
TOPOLOGY [LARGE SCALE ANALYSIS].
DOI=10.1073/pnas.0604075103; PubMed=16847258 [NCBI, ExPASy, EBI, Israel, Japan]
Kim H., Melen K., Oesterberg M., von Heijne G.;
"A global topology map of the Saccharomyces cerevisiae membrane proteome.";
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
S58126; AAD13895.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S57891; AAB19615.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z72534; CAA96712.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S64014; S64014.
RefSeq NP_011503.1; -.
3D structure databases
ModBase P25340.
Protein-protein interaction databases
DIP DIP:7177N; -.
Organism-specific databases
CYGD YGL012w; -.
SGD S000002980; ERG4.
Yeast-GFP YGL012W.
Gene expression databases
ArrayExpress P25340; -.
GermOnline YGL012W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005783; Cellular component: endoplasmic reticulum (inferred from direct assay from SGD).
GO:0000246; Molecular function: delta24(24-1) sterol reductase activity (traceable author statement from SGD).
GO:0006696; Biological process: ergosterol biosynthetic process (traceable author statement from SGD).
QuickGo view.
Family and domain databases
InterPro IPR001171; ERG4_ERG24.
Graphical view of domain structure.
Pfam PF01222; ERG4_ERG24; 1.
Pfam graphical view of domain structure.
PROSITE PS01017; STEROL_REDUCT_1; 1.
PS01018; STEROL_REDUCT_2; 1.
BLOCKS P25340.
Proteomic databases
PeptideAtlas P25340; -.
Genome annotation databases
Ensembl YGL012W; Saccharomyces cerevisiae. [Contig view]
GeneID 852872; -.
GenomeReviews Y13135_GR; YGL012W.
KEGG sce:YGL012W; -.
NMPDR fig|4932.3.peg.2611; -.
Phylogenomic databases
HOGENOM P25340; -.
Other
LinkHub P25340; -.
ProtoNet P25340.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   473  473     Delta(24(24(1)))-sterol reductase. PRO_0000207491
TOPO_DOM   1    39  39     Lumenal (Potential). 
TRANSMEM   40    64  25     Potential. 
TOPO_DOM   65   101  37     Cytoplasmic (Potential). 
TRANSMEM   102   123  22     Potential. 
TOPO_DOM   124   138  15     Lumenal (Potential). 
TRANSMEM   139   157  19     Potential. 
TOPO_DOM   158   174  17     Cytoplasmic (Potential). 
TRANSMEM   175   197  23     Potential. 
TOPO_DOM   198   232  35     Lumenal (Potential). 
TRANSMEM   233   249  17     Potential. 
TOPO_DOM   250   326  77     Cytoplasmic (Potential). 
TRANSMEM   327   346  20     Potential. 
TOPO_DOM   347   414  68     Lumenal (Potential). 
TRANSMEM   415   439  25     Potential. 
TOPO_DOM   440   473  34     Cytoplasmic (Potential). 
CONFLICT   366   366        G -> V (in Ref. 1 and 2). 
Sequence information
Length: 473 AA [This is the length of the unprocessed precursor] Molecular weight: 56040 Da [This is the MW of the unprocessed precursor] CRC64: B4BFF14559272DD6 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAKDNSEKLQ VQGEEKKSKQ PVNFLPQGKW LKPNEIEYEF GGTTGVIGML IGFPLLMYYM 

        70         80         90        100        110        120 
WICAEFYHGK VALPKAGESW MHFIKHLYQL VLENGIPEKY DWTIFLTFWV FQIIFYYTLP 

       130        140        150        160        170        180 
GIWTKGQPLS HLKGKQLPYF CNAMWTLYVT TTLVLVLHFT NLFRLYVIID RFGRIMTCAI 

       190        200        210        220        230        240 
ISGFAFSIIL YLWTLFISHD YHRMTGNHLY DFFMGAPLNP RWGILDLKMF FEVRLPWFTL 

       250        260        270        280        290        300 
YFITLGACLK QWETYGYVTP QLGVVMLAHW LYANACAKGE ELIVPTWDMA YEKFGFMLIF 

       310        320        330        340        350        360 
WNIAGVPYTY CHCTLYLYYH DPSEYHWSTL YNVSLYVVLL CAYYFFDTAN AQKNAFRKQM 

       370        380        390        400        410        420 
SGDKTGRKTF PFLPYQILKN PKYMVTSNGS YLLIDGWYTL ARKIHYTADW TQSLVWALSC 

       430        440        450        460        470 
GFNSVFPWFF PVFFLVVLIH RAFRDQAKCK RKYGKDWDEY CKHCPYVFIP YVF 

P25340 in FASTA format

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