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UniProtKB/Swiss-Prot entry P25688


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name URIC_MOUSE
Primary accession number P25688
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 72)
Name and origin of the protein
Protein name Uricase
Synonyms EC 1.7.3.3
Urate oxidase
Gene name
Name: Uox
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2594778 [NCBI, ExPASy, EBI, Israel, Japan]
Wu X., Lee C.C., Muzny D.M., Caskey C.T.;
"Urate oxidase: primary structure and evolutionary implications.";
Proc. Natl. Acad. Sci. U.S.A. 86:9412-9416(1989).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=FVB/N;
TISSUE=Liver;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
PROTEIN SEQUENCE OF 2-18; 42-52; 56-80; 86-107; 148-158; 165-185; 188-196; 203-214 AND 221-236, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
STRAIN=C57BL/6;
TISSUE=Liver;
Kanor S., Bienvenut W.V.;
Submitted (OCT-2005) to UniProtKB.
[4]
ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-36; LYS-55; LYS-118; LYS-122; LYS-164; LYS-185; LYS-220; LYS-227; LYS-277 AND LYS-290, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1016/j.molcel.2006.06.026; PubMed=16916647 [NCBI, ExPASy, EBI, Israel, Japan]
Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y.;
"Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.";
Mol. Cell 23:607-618(2006).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-231 AND SER-301, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1074/mcp.M600218-MCP200; PubMed=17208939 [NCBI, ExPASy, EBI, Israel, Japan]
Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y.;
"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS.";
Mol. Cell. Proteomics 6:669-676(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M27695; AAA40538.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC019771; AAH19771.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR B36227; B36227.
RefSeq NP_033500.1; -.
UniGene Mm.10865
3D structure databases
HSSP Q00511; 1UOX. [HSSP ENTRY / PDB]
ModBase P25688.
PTM databases
PhosphoSite P25688; -.
2D gel databases
SWISS-2DPAGE P25688; -.
Organism-specific databases
MGI MGI:98907; Uox.
Gene expression databases
ArrayExpress P25688; -.
CleanEx MM_UOX; -.
GermOnline ENSMUSG00000028186; Mus musculus.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from MGI).
GO:0005777; Cellular component: peroxisome (traceable author statement from MGI).
GO:0004846; Molecular function: urate oxidase activity (traceable author statement from MGI).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006144; Biological process: purine base metabolic process (traceable author statement from MGI).
QuickGo view.
Family and domain databases
InterPro IPR002042; Uricase.
Graphical view of domain structure.
Gene3D G3DSA:3.10.270.10; Uricase; 1.
PANTHER PTHR10395:SF1; Uricase; 1.
Pfam PF01014; Uricase; 2.
Pfam graphical view of domain structure.
PRINTS PR00093; URICASE.
ProDom PD003367; Uricase; 2.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR03383; urate_oxi; 1.
PROSITE PS00366; URICASE; 1.
ProtoNet P25688.
Genome annotation databases
Ensembl ENSMUSG00000028186; Mus musculus. [Contig view]
GeneID 22262; -.
KEGG mmu:22262; -.
Phylogenomic databases
HOGENOM P25688; -.
HOVERGEN P25688; -.
Other
NextBio 302359; -.
SOURCE Uox; Mus musculus.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Direct protein sequencing; Oxidoreductase; Peroxisome; Phosphoprotein; Purine metabolism.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   303  302     Uricase. PRO_0000165986
MOTIF   301   303  3     Microbody targeting signal (Potential). 
ACT_SITE   187   187        Charge relay system (By similarity). 
ACT_SITE   235   235        Charge relay system (By similarity). 
MOD_RES   2     2        N-acetylalanine. 
MOD_RES   36    36        N6-acetyllysine. 
MOD_RES   39    39        Phosphoserine. 
MOD_RES   55    55        N6-acetyllysine. 
MOD_RES   118   118        N6-acetyllysine. 
MOD_RES   122   122        N6-acetyllysine. 
MOD_RES   164   164        N6-acetyllysine. 
MOD_RES   185   185        N6-acetyllysine. 
MOD_RES   220   220        N6-acetyllysine. 
MOD_RES   227   227        N6-acetyllysine. 
MOD_RES   231   231        Phosphoserine. 
MOD_RES   277   277        N6-acetyllysine. 
MOD_RES   290   290        N6-acetyllysine. 
MOD_RES   301   301        Phosphoserine. 
Sequence information
Length: 303 AA [This is the length of the unprocessed precursor] Molecular weight: 35039 Da [This is the MW of the unprocessed precursor] CRC64: F521383D05370FA2 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAHYHDNYGK NDEVEFVRTG YGKDMVKVLH IQRDGKYHSI KEVATSVQLT LRSKKDYLHG 

        70         80         90        100        110        120 
DNSDIIPTDT IKNTVHVLAK LRGIRNIETF AMNICEHFLS SFNHVTRAHV YVEEVPWKRF 

       130        140        150        160        170        180 
EKNGIKHVHA FIHTPTGTHF CEVEQMRNGP PVIHSGIKDL KVLKTTQSGF EGFLKDQFTT 

       190        200        210        220        230        240 
LPEVKDRCFA TQVYCKWRYQ RRDVDFEAIW GAVRDIVLQK FAGPYDKGEY SPSVQKTLYD 

       250        260        270        280        290        300 
IQVLSLSQLP EIEDMEISLP NIHYFNIDMS KMGLINKEEV LLPLDNPYGK ITGTVKRKLP 


SRL 

P25688 in FASTA format

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