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UniProtKB/Swiss-Prot entry P25856


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name G3PA_ARATH
Primary accession number P25856
Secondary accession numbers Q41184 Q9LSE6
Integrated into Swiss-Prot on May 1, 1992
Sequence was last modified on May 2, 2002 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 85)
Name and origin of the protein
Protein name Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic [Precursor]
Synonyms EC 1.2.1.13
NADP-dependent glyceraldehydephosphate dehydrogenase subunit A
Gene name
Name: GAPA
OrderedLocusNames: At3g26650
ORFNames: MLJ15.4, MLJ15_5
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
DOI=10.1016/0378-1119(91)90242-4; PubMed=1916285 [NCBI, ExPASy, EBI, Israel, Japan]
Shih M.-C., Heinrich P., Goodman H.M.;
"Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana.";
Gene 104:133-138(1991).
[2]
ERRATUM.
DOI=10.1016/0378-1119(92)90290-6; PubMed=1398114 [NCBI, ExPASy, EBI, Israel, Japan]
Shih M.-C., Heinrich P., Goodman H.M.;
Gene 119:317-319(1992).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
STRAIN=cv. Columbia;
TISSUE=Shoot;
DOI=10.1093/nar/24.21.4313; PubMed=8932388 [NCBI, ExPASy, EBI, Israel, Japan]
Quigley F., Dao P., Cottet A., Mache R.;
"Sequence analysis of an 81 kb contig from Arabidopsis thaliana chromosome III.";
Nucleic Acids Res. 24:4313-4318(1996).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.2.131; PubMed=10819329 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:131-135(2000).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[6]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M300030-MCP200; PubMed=12766230 [NCBI, ExPASy, EBI, Israel, Japan]
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.;
"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana.";
Mol. Cell. Proteomics 2:325-345(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M64114; AAD10209.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M64117; AAA32793.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S45910; AAB23532.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X98130; CAA66816.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB026648; BAB01730.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY058140; AAL25556.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY058107; AAL24215.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF428431; AAL16200.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY075637; AAL91645.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY142053; AAM98317.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JQ1285; JQ1285.
RefSeq NP_566796.2; -.
UniGene At.23357
3D structure databases
HSSP P19866; 1NBO. [HSSP ENTRY / PDB]
SMR P25856; 61-396.
ModBase P25856.
Protein-protein interaction databases
IntAct P25856; -.
Enzyme and pathway databases
BioCyc MetaCyc:AT3G26650-MON; -.
2D gel databases
SWISS-2DPAGE P25856; -.
Organism-specific databases
TAIR At3g26650; -.
Gene expression databases
ArrayExpress P25856; -.
GermOnline AT3G26650; Arabidopsis thaliana.
Family and domain databases
InterPro IPR000173; GlycerAld_3-P_DHase.
IPR006424; Glyceraldehyde-3-P_DHase_1.
Graphical view of domain structure.
PANTHER PTHR10836; GAP_DH; 1.
Pfam PF02800; Gp_dh_C; 1.
PF00044; Gp_dh_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000149; GAP_DH; 1.
PRINTS PR00078; G3PDHDRGNASE.
TIGRFAMs TIGR01534; GAPDH-I; 1.
PROSITE PS00071; GAPDH; 1.
BLOCKS P25856.
Proteomic databases
ProMEX P25856; -.
Genome annotation databases
GeneID 822277; -.
GenomeReviews BA000014_GR; AT3G26650.
KEGG ath:AT3G26650; -.
NMPDR fig|3702.1.peg.14942; -.
Other
ProtoNet P25856.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calvin cycle; Chloroplast; Complete proteome; Membrane; NADP; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
TRANSIT   1    60  60     Chloroplast (By similarity). 
CHAIN   61   396  336     Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic. PRO_0000010416
NP_BIND   71    72  2     NADP (By similarity). 
REGION   212   214  3     Glyceraldehyde 3-phosphate binding (By similarity). 
REGION   271   272  2     Glyceraldehyde 3-phosphate binding (By similarity). 
ACT_SITE   213   213        Nucleophile (By similarity). 
BINDING   95    95        NADP (By similarity). 
BINDING   140   140        NADP; via carbonyl oxygen (By similarity). 
BINDING   243   243        Glyceraldehyde 3-phosphate (By similarity). 
BINDING   258   258        Glyceraldehyde 3-phosphate (By similarity). 
BINDING   294   294        Glyceraldehyde 3-phosphate (By similarity). 
BINDING   376   376        NADP (By similarity). 
SITE   240   240  1     Activates thiol group during catalysis (By similarity). 
CONFLICT   172   172        I -> M (in Ref. 1 and 3). 
Sequence information
Length: 396 AA [This is the length of the unprocessed precursor] Molecular weight: 42490 Da [This is the MW of the unprocessed precursor] CRC64: FFD7FD662FB222FC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MASVTFSVPK GFTEFSGLRS SSASLPFGKK LSSDEFVSIV SFQTSAMGSS GGYRKGVTEA 

        70         80         90        100        110        120 
KLKVAINGFG RIGRNFLRCW HGRKDSPLDI IAINDTGGVK QASHLLKYDS TLGIFDADVK 

       130        140        150        160        170        180 
PSGETAISVD GKIIQVVSNR NPSLLPWKEL GIDIVIEGTG VFVDREGAGK HIEAGAKKVI 

       190        200        210        220        230        240 
ITAPGKGDIP TYVVGVNADA YSHDEPIISN ASCTTNCLAP FVKVLDQKFG IIKGTMTTTH 

       250        260        270        280        290        300 
SYTGDQRLLD ASHRDLRRAR AAALNIVPTS TGAAKAVALV LPNLKGKLNG IALRVPTPNV 

       310        320        330        340        350        360 
SVVDLVVQVS KKTFAEEVNA AFRDSAEKEL KGILDVCDEP LVSVDFRCSD FSTTIDSSLT 

       370        380        390 
MVMGDDMVKV IAWYDNEWGY SQRVVDLADI VANNWK 

P25856 in FASTA format

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