ID   G3PC_MAGLI              Reviewed;         341 AA.
AC   P26518;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-NOV-2008, entry version 53.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, cytosolic;
DE            EC=1.2.1.12;
GN   Name=GAPC; Synonyms=GAPDH;
OS   Magnolia liliiflora.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; magnoliids; Magnoliales; Magnoliaceae;
OC   Magnolia.
OX   NCBI_TaxID=3403;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Martin W., Gierl A., Saedler H.;
RT   "Molecular evidence for pre-Cretaceous angiosperm origins.";
RL   Nature 339:46-48(1989).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; X60347; CAA42905.1; -; mRNA.
DR   PIR; S18483; DEJMG.
DR   HSSP; P56649; 1DSS.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DHase.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DHase_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    341       Glyceraldehyde-3-phosphate dehydrogenase,
FT                                cytosolic.
FT                                /FTId=PRO_0000145604.
FT   NP_BIND      15     16       NAD (By similarity).
FT   REGION      155    157       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      215    216       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    156    156       Nucleophile (By similarity).
FT   BINDING      37     37       NAD (By similarity).
FT   BINDING      84     84       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     186    186       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     238    238       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     320    320       NAD (By similarity).
FT   SITE        183    183       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   341 AA;  36982 MW;  DA1B98CDB9BE2F51 CRC64;
     MGGKKIKIGI NGFGRIGRLV ARVALQRDDV ELVAVNDPFI TTDYMTYMFK YDSVHGQWKH
     HELKVKDSKT LLFGEKPVTV FGVRNPEEIP WGETGAEFVV ESTGVFTDKD KAAAHLKGGA
     KKVIISAPSK DAPMFVVGVN EHEYKSNIDI VSNASCTTNC LAPLAKVIND KFGIVEGLMT
     TVHSITATQK TVDGPSSKDW RGGRAAGFNI IPSSTGAAKA VGKVLPALNG KLTGMAFRVP
     TVDVSVVDLT VRLEKAASYE EIKAVIKAES EGKLKGILGY TEEDVVSTDF IGDNRSSIFD
     AKAGIALNEH FVKLVSWYDN EWGYSSRVID LILIVHMASC Q
//