[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=2037593 [NCBI, ExPASy, EBI, Israel, Japan] Lane B.G., Bernier F., Dratewka-Kos E., Shafai R., Kennedy T.D., Pyne C., Munro J.R., Vaughan T., Walters D., Altomare F.; "Homologies between members of the germin gene family in hexaploid wheat and similarities between these wheat germins and certain Physarum spherulins."; J. Biol. Chem. 266:10461-10469(1991).
[2]	CHARACTERIZATION. PubMed=8509360 [NCBI, ExPASy, EBI, Israel, Japan] Lane B.G., Dunwell J.M., Ray J.A., Schmitt M.R., Cuming A.C.; "Germin, a protein marker of early plant development, is an oxalate oxidase."; J. Biol. Chem. 268:12239-12242(1993).

Comments

FUNCTION: Produces developmental and stress-related release of hydrogen peroxide in the apoplast. May play an important role in several aspects of plant growth and defense mechanisms.
CATALYTIC ACTIVITY: Oxalate + O₂ + 2 H⁺ = 2 CO₂ + H₂O₂.
SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer) (By similarity).
SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast. Cytoplasm. Secreted, cell wall. Note=Found in the apoplast and the cytoplasm of germinating embryo cells. Associated with the cell wall.
MISCELLANEOUS: Associated mostly with highly substituted forms of glucuronogalactoarabinoxylans.
SIMILARITY: Belongs to the germin family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M63224; AAA34271.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P45850; 1FI2. [HSSP ENTRY / PDB]

SMR

P26759; 24-224.

ModBase

P26759.

Organism-specific databases

Gramene

P26759; -.

Ontologies

GO:0048046;	Cellular component: apoplast (inferred from electronic annotation from InterPro).
GO:0005618;	Cellular component: cell wall (inferred from electronic annotation from UniProtKB-KW).
GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0030145;	Molecular function: manganese ion binding (inferred from electronic annotation from InterPro).
GO:0045735;	Molecular function: nutrient reservoir activity (inferred from electronic annotation from InterPro).
GO:0050162;	Molecular function: oxalate oxidase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR006045; Cupin_1.
IPR001929; Germin.
IPR014710; RmlC-like_jellyroll.
Graphical view of domain structure.

Gene3D

G3DSA:2.60.120.10; RmlC-like_jellyroll; 1.

Pfam

PF00190; Cupin_1; 1.
Pfam graphical view of domain structure.

PR00325; GERMIN.

PS00725; GERMIN; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Apoplast; Cell wall; Cytoplasm; Glycoprotein; Manganese; Metal-binding; Oxidoreductase; Secreted; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 23`	`23`	`Potential.`
`CHAIN`	`24 224`	`201`	`Oxalate oxidase GF-3.8.`	`PRO_0000010835`
`METAL`	`111 111`		`Manganese (By similarity).`
`METAL`	`113 113`		`Manganese (By similarity).`
`METAL`	`118 118`		`Manganese (By similarity).`
`METAL`	`160 160`		`Manganese (By similarity).`
`CARBOHYD`	`70 70`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`75 75`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`33 49`		`By similarity.`

Sequence information

Length: 224 AA [This is the length of the unprocessed precursor]

Molecular weight: 23562 Da [This is the MW of the unprocessed precursor]

CRC64: E18A10435FA8BE80 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGYSKNIASG MFAMLLLASA VLSSNPHPLQ DFCVADLDGK AVSVNGHMCK PMSEAGDDFL 

        70         80         90        100        110        120 
FSSKLAKAGN TSTPNGSAVT DLNVAEWPGT NTLGVSMNRV DFAPGGTNPP HIHPRATEIG 

       130        140        150        160        170        180 
IVMKGELLVG ILGSLDSGNK LYSRVVRAGE TFLIPRGLMH FQFNVGKTEA SMVVFFNSQS 

       190        200        210        220 
PSVVFVPLTL FGSNPPIPKP VLTKALRVEA GVVELLKSKF AGGS

P26759 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools