ID   STHA_ECOLI              Reviewed;         466 AA.
AC   P27306; Q2M8Q7;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 5.
DT   25-NOV-2008, entry version 86.
DE   RecName: Full=Soluble pyridine nucleotide transhydrogenase;
DE            Short=STH;
DE            EC=1.6.1.1;
DE   AltName: Full=NAD(P)(+) transhydrogenase [B-specific];
GN   Name=sthA; Synonyms=sth, udhA; OrderedLocusNames=b3962, JW5551;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-27, AND
RP   CHARACTERIZATION.
RX   MEDLINE=99121046; PubMed=9922271;
RA   Boonstra B., French C.E., Wainwright I., Bruce N.C.;
RT   "The udhA gene of Escherichia coli encodes a soluble pyridine
RT   nucleotide transhydrogenase.";
RL   J. Bacteriol. 181:1030-1034(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=94089392; PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA   Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J.,
RA   Daniels D.L.;
RT   "Analysis of the Escherichia coli genome. IV. DNA sequence of the
RT   region from 89.2 to 92.8 minutes.";
RL   Nucleic Acids Res. 21:5408-5417(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-346.
RC   STRAIN=K12;
RX   MEDLINE=93077482; PubMed=1447162;
RA   Gustafsson C., Warne S.R.;
RT   "Physical map of the oxyR-trmA region (minute 89.3) of the Escherichia
RT   coli chromosome.";
RL   J. Bacteriol. 174:7878-7879(1992).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 347-466.
RX   MEDLINE=90066338; PubMed=2511419; DOI=10.1007/BF00332397;
RA   Tao K., Makino K., Yonei S., Nakata A., Shinagawa H.;
RT   "Molecular cloning and nucleotide sequencing of oxyR, the positive
RT   regulatory gene of a regulon for an adaptive response to oxidative
RT   stress in Escherichia coli: homologies between OxyR protein and a
RT   family of bacterial activator proteins.";
RL   Mol. Gen. Genet. 218:371-376(1989).
CC   -!- FUNCTION: Conversion of NADPH, generated by peripheral catabolic
CC       pathways, to NADH, which can enter the respiratory chain for
CC       energy generation.
CC   -!- CATALYTIC ACTIVITY: NADPH + NAD(+) = NADP(+) + NADH.
CC   -!- COFACTOR: Binds 1 FAD per subunit.
CC   -!- SUBUNIT: Homooligomer; probable homooctamer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide
CC       oxidoreductase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC43068.1; Type=Frameshift; Positions=15;
CC       Sequence=CAA46822.1; Type=Frameshift; Positions=15;
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DR   EMBL; U00006; AAC43068.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; U00096; AAC76944.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP009048; BAE77349.1; -; Genomic_DNA.
DR   EMBL; X66026; CAA46822.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; X16531; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; E65203; E65203.
DR   RefSeq; AP_003848.1; -.
DR   RefSeq; NP_418397.2; -.
DR   HSSP; P14218; 1LPF.
DR   GeneID; 948461; -.
DR   GenomeReviews; U00096_GR; b3962.
DR   GenomeReviews; AP009048_GR; JW5551.
DR   KEGG; ecj:JW5551; -.
DR   KEGG; eco:b3962; -.
DR   EchoBASE; EB1398; -.
DR   EcoGene; EG11428; sthA.
DR   HOGENOM; P27306; -.
DR   BioCyc; EcoCyc:UDHA-MON; -.
DR   BioCyc; MetaCyc:UDHA-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0003957; F:NAD(P)+ transhydrogenase (B-specific) activity; IEA:HAMAP.
DR   GO; GO:0045454; P:cell redox homeostasis; IEA:InterPro.
DR   GO; GO:0006739; P:NADP metabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00247; -; 1.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR000815; Hg_reductase.
DR   InterPro; IPR001100; Pyr_nuc-diS_OxRdtase.
DR   InterPro; IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
DR   InterPro; IPR001327; Pyr_OxRdtase_NAD_bd.
DR   Gene3D; G3DSA:3.30.390.30; Pyr_redox_dim; 1.
DR   Pfam; PF00070; Pyr_redox; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   Pfam; PF02852; Pyr_redox_dim; 1.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00945; HGRDTASE.
DR   PRINTS; PR00411; PNDRDTASEI.
DR   ProDom; PD000139; FAD_pyr_redox; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Direct protein sequencing; FAD;
KW   Flavoprotein; NAD; NADP; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    466       Soluble pyridine nucleotide
FT                                transhydrogenase.
FT                                /FTId=PRO_0000068061.
FT   NP_BIND      36     45       FAD (By similarity).
FT   CONFLICT     64     64       I -> L (in Ref. 5; CAA46822).
FT   CONFLICT    118    118       R -> L (in Ref. 5; CAA46822).
SQ   SEQUENCE   466 AA;  51560 MW;  2D7405D1E2D062FD CRC64;
     MPHSYDYDAI VIGSGPGGEG AAMGLVKQGA RVAVIERYQN VGGGCTHWGT IPSKALRHAV
     SRIIEFNQNP LYSDHSRLLR SSFADILNHA DNVINQQTRM RQGFYERNHC EILQGNARFV
     DEHTLALDCP DGSVETLTAE KFVIACGSRP YHPTDVDFTH PRIYDSDSIL SMHHEPRHVL
     IYGAGVIGCE YASIFRGMDV KVDLINTRDR LLAFLDQEMS DSLSYHFWNS GVVIRHNEEY
     EKIEGCDDGV IMHLKSGKKL KADCLLYANG RTGNTDSLAL QNIGLETDSR GQLKVNSMYQ
     TAQPHVYAVG DVIGYPSLAS AAYDQGRIAA QALVKGEATA HLIEDIPTGI YTIPEISSVG
     KTEQQLTAMK VPYEVGRAQF KHLARAQIVG MNVGTLKILF HRETKEILGI HCFGERAAEI
     IHIGQAIMEQ KGGGNTIEYF VNTTFNYPTM AEAYRVAALN GLNRLF
//