ID   DHE2_CLODI              Reviewed;         421 AA.
AC   P27346;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-NOV-2008, entry version 43.
DE   RecName: Full=NAD-specific glutamate dehydrogenase;
DE            Short=NAD-GDH;
DE            EC=1.4.1.2;
GN   Name=gluD;
OS   Clostridium difficile.
OC   Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=1496;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=92129551; PubMed=1774279;
RA   Lyerly D.M., Barroso L.A., Wilkins T.D.;
RT   "Identification of the latex test-reactive protein of Clostridium
RT   difficile as glutamate dehydrogenase.";
RL   J. Clin. Microbiol. 29:2639-2642(1991).
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate
CC       + NH(3) + NADH.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
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DR   EMBL; M65250; AAA62756.1; -; Genomic_DNA.
DR   PIR; S28829; S28829.
DR   HSSP; O74024; 1EUZ.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004352; F:glutamate dehydrogenase activity; IEA:EC.
DR   GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DHase.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DHase_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
DR   InterPro; IPR014362; Glu_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11606:SF2; GLFV_DH; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase.
FT   CHAIN         1    421       NAD-specific glutamate dehydrogenase.
FT                                /FTId=PRO_0000182737.
FT   ACT_SITE    107    107       By similarity.
SQ   SEQUENCE   421 AA;  46016 MW;  31F0A09802F4AD94 CRC64;
     MSGKDVNVFE MAQSQVKNAC DKLGMEPAVY ELLKEPMRVI EVSIPVKMDD GSIKTFKGFR
     SQHNDAVGPT KGGIRFHQNV SRDEVKALSI WMTFKCSVTG IPYGGGKGGI IVDPSTLSQG
     ELERLSRGYI DGIYKLIGEK VDVPAPDVNT NGQIMSWMVD EYNKLTGQSS IGVITGKPVE
     FGGSLGRTAA TGFGVAVTAR EAAAKLGIDM KKAKIAVQGI GNVGSYTVLN CEKLGGTVVA
     MAEWCKSEGS YAIYNENGLD GQAMLDYMKE HGNLLNFPGA KRISLEEFWA SDVDIVIPAA
     LENSITKEVA ESIKAKLVCE AANGPTTPEA DEVFAERGIV LTPDILTNAG GVTVSYFEWV
     QNLYGYYWSE EEVEQKEEIA MVKAFESIWK IKEEYNVTMR EAAYMHSIKK VAEAMKLRGW
     Y
//