ID   DYR_SHV24               Reviewed;         186 AA.
AC   P27421;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-NOV-2008, entry version 47.
DE   RecName: Full=Viral dihydrofolate reductase;
DE            Short=vDHFR;
DE            EC=1.5.1.3;
GN   Name=DHFR; Synonyms=2;
OS   Saimiriine herpesvirus 2 (strain 484-77) (SaHV-2) (Herpesvirus
OS   saimiri).
OC   Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae;
OC   Gammaherpesvirinae; Rhadinovirus.
OX   NCBI_TaxID=10382;
OH   NCBI_TaxID=9521; Saimiri sciureus (Common squirrel monkey).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=94269832; PubMed=8209420; DOI=10.1007/BF01703599;
RA   Geck P., Whitaker S.A., Medveczky M.M., Last T.J., Medveczky P.G.;
RT   "Small RNA expression from the oncogenic region of a highly oncogenic
RT   strain of herpesvirus saimiri.";
RL   Virus Genes 8:25-34(1994).
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X58774; CAA41575.1; -; Genomic_DNA.
DR   HSSP; P00374; 1KMS.
DR   SMR; P27421; 5-185.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001796; DHFR_reg.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   NADP; One-carbon metabolism; Oxidoreductase.
FT   CHAIN         1    186       Viral dihydrofolate reductase.
FT                                /FTId=PRO_0000186379.
FT   DOMAIN        4    184       DHFR.
SQ   SEQUENCE   186 AA;  21674 MW;  8249CD3C7ABE1BA2 CRC64;
     MVLLLNCIVA VDQNMGIGKN GYLPWPLLTN DFKYFQRMTT SSVKNKQNLV IMGKNTWFSI
     PEKNRPLKDR INLVLSKKLK EIPHGAHFLA RSLNDALKLI EQPEFVNKVD MVWIIGGSSV
     YKDAMNYSSH LKLFVTRIMQ SFETDTFFPE IDLKKYKPLI EYPGVPSNTQ EEKGIRYKFE
     VYEKNY
//