ID   DYR_KLEAE               Reviewed;         159 AA.
AC   P27498;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-NOV-2008, entry version 41.
DE   RecName: Full=Dihydrofolate reductase;
DE            EC=1.5.1.3;
GN   Name=folA; Synonyms=atsR;
OS   Klebsiella aerogenes.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Klebsiella.
OX   NCBI_TaxID=28451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W70;
RX   MEDLINE=92202165; PubMed=1551851;
RA   Azakami H., Sugino H., Murooka Y.;
RT   "Cloning and nucleotide sequence of a negative regulator gene for
RT   Klebsiella aerogenes arylsulfatase synthesis and identification of the
RT   gene as folA.";
RL   J. Bacteriol. 174:2344-2351(1992).
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
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DR   EMBL; D10358; BAA01187.1; -; Genomic_DNA.
DR   HSSP; P00379; 1DHI.
DR   SMR; P27498; 1-159.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR001796; DHFR_reg.
DR   PANTHER; PTHR11549:SF1; DHFR; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   NADP; One-carbon metabolism; Oxidoreductase.
FT   CHAIN         1    159       Dihydrofolate reductase.
FT                                /FTId=PRO_0000186392.
FT   DOMAIN        1    158       DHFR.
SQ   SEQUENCE   159 AA;  18112 MW;  BFDC5F9DA53CCE1E CRC64;
     MISLIAALRV DRVIGMENAM PWNLNEDLAW FKRNTLNKPV VMGRLTWESI GRPLPGRKNI
     VISSKPGSDD RVQWVSSVEE AIAACGDVEE IMVIGGGRVY DEFLPKAQKL YLTHIDAEVE
     GDTHFPDYDP DEWESVFSEF HDADAQNSHS YCFEILERR
//