ID   LUCI_RENRE              Reviewed;         311 AA.
AC   P27652;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   04-NOV-2008, entry version 47.
DE   RecName: Full=Renilla-luciferin 2-monooxygenase;
DE            EC=1.13.12.5;
DE   AltName: Full=Renilla-type luciferase;
OS   Renilla reniformis (Sea pansy).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Octocorallia; Pennatulacea;
OC   Sessiliflorae; Renillidae; Renilla.
OX   NCBI_TaxID=6136;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=91239583; PubMed=1674607;
RA   Lorenz W.W., McCann R.O., Longiaru M., Cormier M.J.;
RT   "Isolation and expression of a cDNA encoding Renilla reniformis
RT   luciferase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:4438-4442(1991).
CC   -!- CATALYTIC ACTIVITY: Renilla luciferin + O(2) = oxidized Renilla
CC       luciferin + CO(2) + light.
CC   -!- SUBUNIT: Monomer.
CC   -!- MISCELLANEOUS: This luciferase produces light with a wavelength of
CC       480 nm. In presence of a green fluorescence protein (GFP) it
CC       produces a green fluorescence at 509 nm.
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DR   EMBL; M63501; AAA29804.1; -; mRNA.
DR   PDB; 2PSD; X-ray; 1.40 A; A=3-311.
DR   PDB; 2PSE; X-ray; 2.50 A; A=3-311.
DR   PDB; 2PSF; X-ray; 1.40 A; A/B=3-311.
DR   PDB; 2PSH; X-ray; 1.79 A; A/B=1-311.
DR   PDB; 2PSJ; X-ray; 1.80 A; A/B=1-311.
DR   PDBsum; 2PSD; -.
DR   PDBsum; 2PSE; -.
DR   PDBsum; 2PSF; -.
DR   PDBsum; 2PSH; -.
DR   PDBsum; 2PSJ; -.
DR   GO; GO:0016831; F:carboxy-lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0050248; F:Renilla-luciferin 2-monooxygenase activity; IEA:EC.
DR   GO; GO:0008218; P:bioluminescence; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00412; EPOXHYDRLASE.
PE   1: Evidence at protein level;
KW   3D-structure; Decarboxylase; Direct protein sequencing; Luminescence;
KW   Lyase; Monooxygenase; Oxidoreductase; Photoprotein.
FT   CHAIN         1    311       Renilla-luciferin 2-monooxygenase.
FT                                /FTId=PRO_0000084523.
FT   CONFLICT    219    219       W -> L (in Ref. 1; AA sequence).
FT   HELIX        10     13
FT   HELIX        17     23
FT   STRAND       25     29
FT   STRAND       32     38
FT   STRAND       45     50
FT   HELIX        57     60
FT   TURN         61     63
FT   HELIX        64     66
FT   TURN         67     70
FT   STRAND       71     76
FT   HELIX        94    105
FT   STRAND      112    119
FT   HELIX       121    132
FT   STRAND      136    144
FT   HELIX       160    167
FT   HELIX       170    175
FT   TURN        176    178
FT   HELIX       180    183
FT   HELIX       185    188
FT   HELIX       196    203
FT   HELIX       204    206
FT   STRAND      208    210
FT   HELIX       211    213
FT   HELIX       214    221
FT   TURN        226    228
FT   HELIX       231    245
FT   STRAND      252    259
FT   HELIX       263    270
FT   STRAND      273    286
FT   HELIX       287    289
FT   HELIX       292    307
SQ   SEQUENCE   311 AA;  36022 MW;  0A3FD025B4EC33FD CRC64;
     MTSKVYDPEQ RKRMITGPQW WARCKQMNVL DSFINYYDSE KHAENAVIFL HGNAASSYLW
     RHVVPHIEPV ARCIIPDLIG MGKSGKSGNG SYRLLDHYKY LTAWFELLNL PKKIIFVGHD
     WGACLAFHYS YEHQDKIKAI VHAESVVDVI ESWDEWPDIE EDIALIKSEE GEKMVLENNF
     FVETMLPSKI MRKLEPEEFA AYLEPFKEKG EVRRPTLSWP REIPLVKGGK PDVVQIVRNY
     NAYLRASDDL PKMFIESDPG FFSNAIVEGA KKFPNTEFVK VKGLHFSQED APDEMGKYIK
     SFVERVLKNE Q
//