ID   P5CR_TREPA              Reviewed;         263 AA.
AC   P27771; O83775;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   25-NOV-2008, entry version 63.
DE   RecName: Full=Pyrroline-5-carboxylate reductase;
DE            Short=P5C reductase;
DE            Short=P5CR;
DE            EC=1.5.1.2;
GN   Name=proC; OrderedLocusNames=TP_0797;
OS   Treponema pallidum.
OC   Bacteria; Spirochaetes; Spirochaetales; Spirochaetaceae; Treponema.
OX   NCBI_TaxID=160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Nichols;
RX   MEDLINE=90264287; PubMed=2188947;
RA   Gherardini F.C., Hobbs M.M., Stamm L.V., Bassford P.J. Jr.;
RT   "Complementation of an Escherichia coli proC mutation by a gene cloned
RT   from Treponema pallidum.";
RL   J. Bacteriol. 172:2996-3002(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nichols;
RX   MEDLINE=98332770; PubMed=9665876; DOI=10.1126/science.281.5375.375;
RA   Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A.,
RA   Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D.,
RA   Khalak H.G., Richardson D.L., Howell J.K., Chidambaram M.,
RA   Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D.,
RA   Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M.,
RA   Weidman J.F., Smith H.O., Venter J.C.;
RT   "Complete genome sequence of Treponema pallidum, the syphilis
RT   spirochete.";
RL   Science 281:375-388(1998).
CC   -!- CATALYTIC ACTIVITY: L-proline + NAD(P)(+) = 1-pyrroline-5-
CC       carboxylate + NAD(P)H.
CC   -!- PATHWAY: Amino-acid biosynthesis; L-proline biosynthesis; L-
CC       proline from L-glutamate 5-semialdehyde: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the pyrroline-5-carboxylate reductase
CC       family.
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DR   EMBL; M73825; AAA27478.1; -; Genomic_DNA.
DR   EMBL; AE000520; AAC65760.1; -; Genomic_DNA.
DR   PIR; D71281; D71281.
DR   RefSeq; NP_219234.1; -.
DR   IntAct; P27771; -.
DR   GeneID; 2610812; -.
DR   GenomeReviews; AE000520_GR; TP_0797.
DR   KEGG; tpa:TP0797; -.
DR   NMPDR; fig|243276.1.peg.793; -.
DR   TIGR; TP_0797; -.
DR   HOGENOM; P27771; -.
DR   BioCyc; TPAL243276:TP_0797-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004735; F:pyrroline-5-carboxylate reductase activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006561; P:proline biosynthetic process; IEA:InterPro.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   InterPro; IPR004455; NADP_OxRdtase_F420.
DR   InterPro; IPR000304; P5CR.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11645; P5CR; 1.
DR   Pfam; PF03807; F420_oxidored; 1.
DR   PIRSF; PIRSF000193; Pyrrol-5-carb_rd; 1.
DR   TIGRFAMs; TIGR00112; proC; 1.
DR   PROSITE; PS00521; P5CR; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; Complete proteome; Cytoplasm; NADP;
KW   Oxidoreductase; Proline biosynthesis.
FT   CHAIN         1    263       Pyrroline-5-carboxylate reductase.
FT                                /FTId=PRO_0000187310.
FT   CONFLICT      1     65       MNVGFLGFGAMGRALAEGLVHAGALQAAQVYACALNQEKLR
FT                                AQCTSLGIGACASVQELVQKSEWI -> MTWDFWVLEQWDG
FT                                RWQKGWCTQERCKRSSVRLCVKSGKVACAVYIFGHRCLRVS
FT                                SGTGTEKVNGF (in Ref. 1).
FT   CONFLICT     77     93       VLRDRQSFQGKVLISLA -> GTARSPIFQESAISC (in
FT                                Ref. 1; AAA27478).
FT   CONFLICT    256    260       VRAAL -> CRWLS (in Ref. 1).
SQ   SEQUENCE   263 AA;  27645 MW;  ED4AD7C54BAF9D61 CRC64;
     MNVGFLGFGA MGRALAEGLV HAGALQAAQV YACALNQEKL RAQCTSLGIG ACASVQELVQ
     KSEWIFLAVK PSQISTVLRD RQSFQGKVLI SLAAGMSCAA YEALFAADPH QGIRHLSLLP
     NLPCQVARGV IIAEARHTLH HDEHAALLAV LRTVAQVEVV DTAYFAIAGV IAGCAPAFAA
     QFIEALADAG VRYGLARDQA YRLAAHMLEG TAALIQHSGV HPAQLKDRVC SPAGSTIRGV
     LALEEQGLRR AVIHAVRAAL SSS
//