NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 34-125; 133-154; 157-200 AND 203-314, AND FUNCTION.
STRAIN=AKU 4429;
PubMed=8779568 [NCBI, ExPASy, EBI, Israel, Japan]
Kita K., Matsuzaki K., Hashimoto T., Yanase H., Kato N., Chung M.C.-M., Kataoka M., Shimizu S.;
"Cloning of the aldehyde reductase gene from a red yeast, Sporobolomyces salmonicolor, and characterization of the gene and its product.";
Appl. Environ. Microbiol. 62:2303-2310(1996).

[2]

PROTEIN SEQUENCE OF 2-52, AND ENZYME REGULATION.
STRAIN=AKU 4429;
DOI=10.1016/0167-4838(92)90127-Y; PubMed=1633196 [NCBI, ExPASy, EBI, Israel, Japan]
Kataoka M., Sakai H., Morikawa T., Katoh M., Miyoshi T., Shimizu S., Yamada H.;
"Characterization of aldehyde reductase of Sporobolomyces salmonicolor.";
Biochim. Biophys. Acta 1122:57-62(1992).

Comments

FUNCTION: Catalyzes the asymmetric reduction of aliphatic and aromatic aldehydes and ketones to an R-enantiomer. Reduces ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate.
CATALYTIC ACTIVITY: An alcohol + NADP⁺ = an aldehyde + NADPH.
ENZYME REGULATION: Inhibited by quercetin, dicoumarol and some SH-reagents.
SUBUNIT: Monomer.
SIMILARITY: Belongs to the aldo/keto reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U26463; AAB17362.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S78113; S78113.

3D structure databases

HSSP

P14550; 2ALR. [HSSP ENTRY / PDB]

ModBase

P27800.

Ontologies

GO:0005623;	Cellular component: cell (inferred from direct assay from UniProtKB).
GO:0008106;	Molecular function: alcohol dehydrogenase (NADP+) activity (inferred from direct assay from UniProtKB).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001395; Aldo/ket_red.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.100; Aldo/ket_red; 1.

PANTHER

PTHR11732; Aldo/ket_red; 1.

Pfam

PF00248; Aldo_ket_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00069; ALDKETRDTASE.

ProDom

PD000288; Aldo/ket_red; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00798; ALDOKETO_REDUCTASE_1; 1.
PS00062; ALDOKETO_REDUCTASE_2; 1.
PS00063; ALDOKETO_REDUCTASE_3; FALSE_NEG.

ProtoNet

P27800.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 323`	`322`	`Aldehyde reductase 1.`	`PRO_0000124621`
`NP_BIND`	`210 267`	`58`	`NADP (By similarity).`
`ACT_SITE`	`49 49`		`Proton donor (By similarity).`
`BINDING`	`107 107`		`Substrate (By similarity).`
`SITE`	`74 74`	`1`	`Lowers pKa of active site Tyr (By similarity).`

Sequence information

Length: 323 AA [This is the length of the unprocessed precursor]

Molecular weight: 35339 Da [This is the MW of the unprocessed precursor]

CRC64: D49B33ACB41D4D60 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVGTTTLNTG ASLELVGYGT WQAAPGEVGQ GVKVAIETGY RHLDLAKVYS NQPEVGAAIK 

        70         80         90        100        110        120 
EAGVKREDLF ITSKLWNNSH RPEQVEPALD DTLKELGLEY LDLYLIHWPV AFPPEGDITQ 

       130        140        150        160        170        180 
NLFPKANDKE VKLDLEVSLV DTWKAMVKLL DTGKVKAIGV SNFDAKMVDA IIEATGVTPS 

       190        200        210        220        230        240 
VNQIERHPLL LQPELIAHHK AKNIHITAYS PLGNNTVGAP LLVQHPEIKR IAEKNGCTPA 

       250        260        270        280        290        300 
QVLIAWAIVG GHSVIPKSVT PSRIGENFKQ VSLSQEDVDA VSKLGEGSGR RRYNIPCTYS 

       310        320 
PKWDINVFGE EDEKSCKNAV KIK

P27800 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools