ID   DHSO_RAT                Reviewed;         357 AA.
AC   P27867; A2VCV9; Q4FZY4; Q5I0F3;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 4.
DT   25-NOV-2008, entry version 89.
DE   RecName: Full=Sorbitol dehydrogenase;
DE            EC=1.1.1.14;
DE   AltName: Full=L-iditol 2-dehydrogenase;
GN   Name=Sord; Synonyms=Sdh1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   MEDLINE=91266961; PubMed=2050152;
RA   Karlsson C., Joernvall H., Heoeog J.O.;
RT   "Sorbitol dehydrogenase: cDNA coding for the rat enzyme. Variations
RT   within the alcohol dehydrogenase family independent of quaternary
RT   structure and metal content.";
RL   Eur. J. Biochem. 198:761-765(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Testis;
RX   MEDLINE=94039079; PubMed=8223590;
RA   Wen Y., Bekhor I.;
RT   "Sorbitol dehydrogenase. Full-length cDNA sequencing reveals a mRNA
RT   coding for a protein containing an additional 42 amino acids at the N-
RT   terminal end.";
RL   Eur. J. Biochem. 217:83-87(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryonic liver, Liver, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Liver;
RX   PubMed=16396499; DOI=10.1021/pr0503073;
RA   Moser K., White F.M.;
RT   "Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-
RT   MS/MS.";
RL   J. Proteome Res. 5:98-104(2006).
CC   -!- CATALYTIC ACTIVITY: L-iditol + NAD(+) = L-sorbose + NADH.
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
CC   -!- CAUTION: PubMed:8223590 reports a cDNA predicted to encode a
CC       protein with an extended N-terminus but there is no further
CC       evidence for the existence of such a protein.
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DR   EMBL; X59037; CAA41761.1; -; mRNA.
DR   EMBL; X74593; CAA52670.1; ALT_INIT; mRNA.
DR   EMBL; BC088398; AAH88398.2; -; mRNA.
DR   EMBL; BC098919; AAH98919.2; -; mRNA.
DR   EMBL; BC128707; AAI28708.2; -; mRNA.
DR   PIR; S38363; S16132.
DR   RefSeq; NP_058748.2; -.
DR   UniGene; Rn.11334; -.
DR   HSSP; O96496; 1E3J.
DR   SMR; P27867; 44-399.
DR   PhosphoSite; P27867; -.
DR   Ensembl; ENSRNOG00000017291; Rattus norvegicus.
DR   GeneID; 24788; -.
DR   KEGG; rno:24788; -.
DR   NMPDR; fig|10116.3.peg.19584; -.
DR   RGD; 3734; Sord.
DR   HOVERGEN; P27867; -.
DR   NextBio; 604416; -.
DR   GermOnline; ENSRNOG00000017291; Rattus norvegicus.
DR   GO; GO:0003939; F:L-iditol 2-dehydrogenase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:InterPro.
DR   InterPro; IPR013154; AlcDHase_GroES-like.
DR   InterPro; IPR002085; AlcDHase_SF_Zn.
DR   InterPro; IPR013149; AlcDHase_Zn-bd.
DR   InterPro; IPR002328; AlcDHase_Zn_CS.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   ProDom; PD040557; GroES_related; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Metal-binding; NAD; Oxidoreductase; Phosphoprotein; Zinc.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    357       Sorbitol dehydrogenase.
FT                                /FTId=PRO_0000000884.
FT   METAL        45     45       Zinc; catalytic (By similarity).
FT   METAL        70     70       Zinc; catalytic (By similarity).
FT   METAL        71     71       Zinc; catalytic (By similarity).
FT   MOD_RES       2      2       N-acetylalanine (By similarity).
FT   MOD_RES     169    169       Phosphoserine.
FT   CONFLICT    259    259       T -> D (in Ref. 1; CAA41761).
SQ   SEQUENCE   357 AA;  38235 MW;  E6F535775EF73D36 CRC64;
     MAAPAKGENL SLVVHGPGDI RLENYPIPEL GPNDVLLKMH SVGICGSDVH YWEHGRIGDF
     VVKKPMVLGH EAAGTVTKVG PMVKHLKPGD RVAIEPGVPR EIDEFCKIGR YNLTPSIFFC
     ATPPDDGNLC RFYKHSADFC YKLPDSVTFE EGALIEPLSV GIYACRRGSV SLGNKVLVCG
     AGPIGIVTLL VAKAMGASQV VVIDLSASRL AKAKEVGADF TIQVAKETPH DIAKKVESVL
     GSKPEVTIEC TGAESSVQTG IYATHSGGTL VVVGMGPEMI NLPLVHAAVR EVDIKGVFRY
     CNTWPMAVSM LASKTLNVKP LVTHRFPLEK AVEAFETAKK GLGLKVMIKC DPNDQNP
//