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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1748656 [NCBI, ExPASy, EBI, Israel, Japan]
Morton T.A.,
Runquist J.A.,
Ragsdale S.W.,
Shanmugasundaram T.,
Wood H.G.,
Ljungdahl L.G.;
"The primary structure of the subunits of carbon monoxide dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum.";
J. Biol. Chem. 266:23824-23828(1991).
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[2]
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PROTEIN SEQUENCE OF 494-508.
DOI=10.1016/0014-5793(93)81808-D; PubMed=8325380 [NCBI, ExPASy, EBI, Israel, Japan]
Shanmugasundaram T.,
Sundaresh C.S.,
Kumar G.K.;
"Identification of a cysteine involved in the interaction between carbon monoxide dehydrogenase and corrinoid/Fe-S protein from Clostridium thermoaceticum.";
FEBS Lett. 326:281-284(1993).
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[3]
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X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
DOI=10.1126/science.1075843; PubMed=12386327 [NCBI, ExPASy, EBI, Israel, Japan]
Doukov T.I.,
Iverson T.M.,
Seravalli J.,
Ragsdale S.W.,
Drennan C.L.;
"A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase.";
Science 298:567-572(2002).
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[4]
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X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
DOI=10.1038/nsb912; PubMed=12627225 [NCBI, ExPASy, EBI, Israel, Japan]
Darnault C.,
Volbeda A.,
Kim E.J.,
Legrand P.,
Vernede X.,
Lindahl P.A.,
Fontecilla-Camps J.-C.;
"Ni-Zn-[Fe4-S4] and Ni-Ni-[Fe4-S4] clusters in closed and open subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase.";
Nat. Struct. Biol. 10:271-279(2003).
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- FUNCTION: The beta subunit generates CO from CO(2), while the alpha subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the alpha subunit by a corrinoid iron-sulfur protein.
- CATALYTIC ACTIVITY: Acetyl-CoA + corrinoid protein = CO + methylcorrinoid protein + CoA.
- COFACTOR: Binds 2 nickel ions per subunit.
- COFACTOR: Binds 1 4Fe-4S cluster per subunit.
- SUBUNIT: Tetramer of two alpha and two beta chains.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 729 AA [This is the length of the unprocessed precursor] |
Molecular weight: 81725 Da [This is the MW of the unprocessed precursor] |
CRC64: 619BB19D959F5A72 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MTDFDKIFEG AIPEGKEPVA LFREVYHGAI TATSYAEILL NQAIRTYGPD HPVGYPDTAY
70 80 90 100 110 120
YLPVIRCFSG EEVKKLGDLP PILNRKRAQV SPVLNFENAR LAGEATWYAA EIIEALRYLK
130 140 150 160 170 180
YKPDEPLLPP PWTGFIGDPV VRRFGIKMVD WTIPGEAIIL GRAKDSKALA KIVKELMGMG
190 200 210 220 230 240
FMLFICDEAV EQLLEENVKL GIDYIAYPLG NFTQIVHAAN YALRAGMMFG GVTPGAREEQ
250 260 270 280 290 300
RDYQRRRIRA FVLYLGEHDM VKTAAAFGAI FTGFPVITDQ PLPEDKQIPD WFFSVEDYDK
310 320 330 340 350 360
IVQIAMETRG IKLTKIKLDL PINFGPAFEG ESIRKGDMYV EMGGNRTPAF ELVRTVSESE
370 380 390 400 410 420
ITDGKIEVIG PDIDQIPEGS KLPLGILVDI YGRKMQADFE GVLERRIHDF INYGEGLWHT
430 440 450 460 470 480
GQRNINWLRV SKDAVAKGFR FKNYGEILVA KMKEEFPAIV DRVQVTIFTD EAKVKEYMEV
490 500 510 520 530 540
AREKYKERDD RMRGLTDETV DTFYSCVLCQ SFAPNHVCIV TPERVGLCGA VSWLDAKASY
550 560 570 580 590 600
EINHAGPNQP IPKEGEIDPI KGIWKSVNDY LYTASNRNLE QVCLYTLMEN PMTSCGCFEA
610 620 630 640 650 660
IMAILPECNG IMITTRDHAG MTPSGMTFST LAGMIGGGTQ TPGFMGIGRT YIVSKKFISA
670 680 690 700 710 720
DGGIARIVWM PKSLKDFLHD EFVRRSVEEG LGEDFIDKIA DETIGTTVDE ILPYLEEKGH
PALTMDPIM
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P27988 in FASTA format |
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