[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=1915358 [NCBI, ExPASy, EBI, Israel, Japan] Shotkoski F.A., Fallon A.M.; "An amplified insect dihydrofolate reductase gene contains a single intron."; Eur. J. Biochem. 201:157-160(1991).

Comments

CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH.
PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1 .
MISCELLANEOUS: The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
SIMILARITY: Belongs to the dihydrofolate reductase family.
SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X60192; CAA42748.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S17984; S17984.

3D structure databases

HSSP

P00378; 8DFR. [HSSP ENTRY / PDB]

ModBase

P28019.

Family and domain databases

InterPro

IPR012259; DHFR.
IPR001796; DHFR_reg.
Graphical view of domain structure.

PANTHER

PTHR11549:SF1; DHFR; 1.

Pfam

PF00186; DHFR_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00070; DHFR.

PROSITE

PS00075; DHFR_1; 1.
PS51330; DHFR_2; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

NADP; One-carbon metabolism; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 186`	`186`	`Dihydrofolate reductase.`	`PRO_0000186369`
`DOMAIN`	`3 183`	`181`	`DHFR.`

Sequence information

Length: 186 AA [This is the length of the unprocessed precursor]

Molecular weight: 21447 Da [This is the MW of the unprocessed precursor]

CRC64: AFCD7F469779D83C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKFSLIVAV CANGGIGIKG DLPWRLRQEL KYFSRMTKKI QDSGKRNAII MGRKTYFGVP 

        70         80         90        100        110        120 
ESKRPLPERL NIILTRDPSA NAYPSEVMVC TSMQEALKKL DEAPLVNEIE NVWIVGGNAV 

       130        140        150        160        170        180 
YKEAMQSDRC HRIYLTEIKE TFECDAFFPE ITSDFQLVKN DDDVPEDIQE ENGIQYQYRI 


YEKVPK

P28019 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools