ID   DYR_AEDAL               Reviewed;         186 AA.
AC   P28019;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-NOV-2008, entry version 43.
DE   RecName: Full=Dihydrofolate reductase;
DE            EC=1.5.1.3;
GN   Name=DHFR;
OS   Aedes albopictus (Forest day mosquito).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Culicinae; Culicini; Aedes; Stegomyia.
OX   NCBI_TaxID=7160;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=92007869; PubMed=1915358;
RA   Shotkoski F.A., Fallon A.M.;
RT   "An amplified insect dihydrofolate reductase gene contains a single
RT   intron.";
RL   Eur. J. Biochem. 201:157-160(1991).
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
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DR   EMBL; X60192; CAA42748.1; -; Genomic_DNA.
DR   PIR; S17984; S17984.
DR   HSSP; P00378; 8DFR.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR001796; DHFR_reg.
DR   PANTHER; PTHR11549:SF1; DHFR; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   NADP; One-carbon metabolism; Oxidoreductase.
FT   CHAIN         1    186       Dihydrofolate reductase.
FT                                /FTId=PRO_0000186369.
FT   DOMAIN        3    183       DHFR.
SQ   SEQUENCE   186 AA;  21447 MW;  AFCD7F469779D83C CRC64;
     MKKFSLIVAV CANGGIGIKG DLPWRLRQEL KYFSRMTKKI QDSGKRNAII MGRKTYFGVP
     ESKRPLPERL NIILTRDPSA NAYPSEVMVC TSMQEALKKL DEAPLVNEIE NVWIVGGNAV
     YKEAMQSDRC HRIYLTEIKE TFECDAFFPE ITSDFQLVKN DDDVPEDIQE ENGIQYQYRI
     YEKVPK
//