[1]	NUCLEOTIDE SEQUENCE. PubMed=1559991 [NCBI, ExPASy, EBI, Israel, Japan] Yee J., Dennis P.P.; "Isolation and characterization of a NADP-dependent glutamate dehydrogenase gene from the primitive eucaryote Giardia lamblia."; J. Biol. Chem. 267:7539-7544(1992).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-261. PubMed=8587793 [NCBI, ExPASy, EBI, Israel, Japan] Monis P.T., Mayrhofer G., Andrews R.H., Homan W.L., Limper L., Ey P.L.; "Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus."; Parasitology 112:1-12(1996).

Comments

CATALYTIC ACTIVITY: L-glutamate + H₂O + NADP⁺ = 2-oxoglutarate + NH₃ + NADPH.
SUBUNIT: Homohexamer (By similarity).
SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M84604; AAA29155.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U47632; AAB05400.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A42489; A42489.

3D structure databases

HSSP

P24295; 1AUP. [HSSP ENTRY / PDB]

ModBase

P28724.

Family and domain databases

InterPro

IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11606:SF2; GLFV_DH; 1.

Pfam

PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000185; Glu_DH; 1.

PRINTS

PR00082; GLFDHDRGNASE.

PROSITE

PS00074; GLFV_DEHYDROGENASE; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 449`	`449`	`NADP-specific glutamate dehydrogenase.`	`PRO_0000182786`
`ACT_SITE`	`125 125`		`By similarity.`
`CONFLICT`	`249 249`		`I -> L (in Ref. 2; AAB05400).`

Sequence information

Length: 449 AA [This is the length of the unprocessed precursor]

Molecular weight: 49766 Da [This is the MW of the unprocessed precursor]

CRC64: 5497B35209B549F6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPAQTIEELI AVIKQRDGHM TEFRQAVEEV VDSLKVIFER EPKYIPIFER MLEPERVIIF 

        70         80         90        100        110        120 
RVPWMDDAGR INVNRGFRVQ YNSALGPYKG GLRFHPSVNL SILKFLGFEQ ILKNSLTTLP 

       130        140        150        160        170        180 
MGGGKGGSDF DPKGKSDNEV MRFCQSFMTE LQRHVGADTD VPAGDIGVGA REIGYLYGQY 

       190        200        210        220        230        240 
KRLRNEFTGV LTGKNVKWGG SFIRPEATGY GAVYFLEEMC KDNNTVIRGK NVLLSGSGNV 

       250        260        270        280        290        300 
AQFACEKLIQ LGAKVLTFSD SNGTIVDKDG FNEEKLAHLM YLKNEKRGRV SEFKDKYPSV 

       310        320        330        340        350        360 
AYYEGKKPWE CFEGQMDCIM PCATQNEVSG DDATRLVGLG LKFVAEGANM PSTAEAVHVY 

       370        380        390        400        410        420 
HAKGVMYGPA KASNAGGVSV SGLEMSQNSV RLQWTAEEVD QKLRGIMRGI FVACRDTAKK 

       430        440 
YGHPKNYQMG ANIAGFLKVA DSMIEQGCV

P28724 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools