NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
DOI=10.1007/BF00037142; PubMed=1718478 [NCBI, ExPASy, EBI, Israel, Japan]
Cock J.M., Kim K.D., Miller P.W., Hutson R.G., Schmidt R.R.;
"A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana.";
Plant Mol. Biol. 17:1023-1044(1991).

Comments

CATALYTIC ACTIVITY: L-glutamate + H₂O + NADP⁺ = 2-oxoglutarate + NH₃ + NADPH.
SUBUNIT: Homo- and heterohexamer of alpha and beta subunits. Both subunits are encoded by the same gene.
SUBCELLULAR LOCATION: Plastid, chloroplast.
INDUCTION: By ammonium.
PTM: The N-termini of the alpha and the beta chains are blocked.
SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X58832; CAA41636.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X58831; CAA41635.1; ALT_SEQ; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S17949; S17949.

3D structure databases

HSSP

P24295; 1AUP. [HSSP ENTRY / PDB]

ModBase

P28998.

Family and domain databases

InterPro

IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR11606:SF2; GLFV_DH; 1.

Pfam

PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000185; Glu_DH; 1.

PRINTS

PR00082; GLFDHDRGNASE.

PROSITE

PS00074; GLFV_DEHYDROGENASE; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chloroplast; NADP; Oxidoreductase; Plastid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 523`	`>523`	`NADP-specific glutamate dehydrogenase.`	`PRO_0000182782`
`ACT_SITE`	`202 202`		`By similarity.`
`NON_TER`	`1 1`

Sequence information

Length: 523 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 57530 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: A35FE730E5FEF974 [This is a checksum on the sequence]

        10         20         30         40         50         60 
CRPPSSPSLS WPPGLRSPAL PRAVACARGR SAKRDVAAKR LRSRSPRMDA TTGDFTALQK 

        70         80         90        100        110        120 
AVKQMATKAG TEGLVHGIKN PELRQLLTEI FMKDPEQQEF MQAVREVAVS LQPVFEKRPE 

       130        140        150        160        170        180 
LLPIFKQIVE PERVITFRVS WLDDAGNLQV NRGFRVQYSS AIGPYKGGLR FHPSVNLSIM 

       190        200        210        220        230        240 
KFLAFEQIFK NSLTTLPMGG GKGGSDFDPK GKSDAEVMRF CQSFMTELQR HISYVQDVPA 

       250        260        270        280        290        300 
GDIGVGAREI GYLFGQYKRI TKNYTGVLTG KGQEYGGSEI RPEATGYGAV LFVENVLKDK 

       310        320        330        340        350        360 
GESLKGKRCL VSGAGNVAQY CAELLLEKGA IVLSLSDSQG YVYEPNGFTR EQLQAVQDMK 

       370        380        390        400        410        420 
KKNNSARISE YKSDTAVYVG DRRKPWELDC QVDIAFPCAT QNEIDEHDAE LLIKHGCQYV 

       430        440        450        460        470        480 
VEGANMPSTN EAIHKYNKAG IIYCPGKAAN AGGVAVSGLE MTQNRMSLNW TREEVRDKLE 

       490        500        510        520 
RIMKDIYDSA MGPSREYNVD LAAGANIAGF TKVADAVKAQ GAV

P28998 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools