ID   DHE4_CHLSO              Reviewed;         523 AA.
AC   P28998;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-NOV-2008, entry version 47.
DE   RecName: Full=NADP-specific glutamate dehydrogenase;
DE            Short=NADP-GDH;
DE            EC=1.4.1.4;
DE   Flags: Fragment;
OS   Chlorella sorokiniana.
OC   Eukaryota; Viridiplantae; Chlorophyta; Trebouxiophyceae; Chlorellales;
OC   Chlorellaceae; Chlorella.
OX   NCBI_TaxID=3076;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RX   MEDLINE=92032762; PubMed=1718478; DOI=10.1007/BF00037142;
RA   Cock J.M., Kim K.D., Miller P.W., Hutson R.G., Schmidt R.R.;
RT   "A nuclear gene with many introns encoding ammonium-inducible
RT   chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella
RT   sorokiniana.";
RL   Plant Mol. Biol. 17:1023-1044(1991).
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate
CC       + NH(3) + NADPH.
CC   -!- SUBUNIT: Homo- and heterohexamer of alpha and beta subunits. Both
CC       subunits are encoded by the same gene.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- INDUCTION: By ammonium.
CC   -!- PTM: The N-termini of the alpha and the beta chains are blocked.
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
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DR   EMBL; X58832; CAA41636.1; -; mRNA.
DR   EMBL; X58831; CAA41635.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S17949; S17949.
DR   HSSP; P24295; 1AUP.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-KW.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DHase.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DHase_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
DR   InterPro; IPR014362; Glu_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11606:SF2; GLFV_DH; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; NADP; Oxidoreductase; Plastid.
FT   CHAIN        <1    523       NADP-specific glutamate dehydrogenase.
FT                                /FTId=PRO_0000182782.
FT   ACT_SITE    202    202       By similarity.
FT   NON_TER       1      1
SQ   SEQUENCE   523 AA;  57530 MW;  A35FE730E5FEF974 CRC64;
     CRPPSSPSLS WPPGLRSPAL PRAVACARGR SAKRDVAAKR LRSRSPRMDA TTGDFTALQK
     AVKQMATKAG TEGLVHGIKN PELRQLLTEI FMKDPEQQEF MQAVREVAVS LQPVFEKRPE
     LLPIFKQIVE PERVITFRVS WLDDAGNLQV NRGFRVQYSS AIGPYKGGLR FHPSVNLSIM
     KFLAFEQIFK NSLTTLPMGG GKGGSDFDPK GKSDAEVMRF CQSFMTELQR HISYVQDVPA
     GDIGVGAREI GYLFGQYKRI TKNYTGVLTG KGQEYGGSEI RPEATGYGAV LFVENVLKDK
     GESLKGKRCL VSGAGNVAQY CAELLLEKGA IVLSLSDSQG YVYEPNGFTR EQLQAVQDMK
     KKNNSARISE YKSDTAVYVG DRRKPWELDC QVDIAFPCAT QNEIDEHDAE LLIKHGCQYV
     VEGANMPSTN EAIHKYNKAG IIYCPGKAAN AGGVAVSGLE MTQNRMSLNW TREEVRDKLE
     RIMKDIYDSA MGPSREYNVD LAAGANIAGF TKVADAVKAQ GAV
//