ID   HMDH1_HEVBR             Reviewed;         575 AA.
AC   P29057;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-NOV-2008, entry version 63.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 1;
DE            Short=HMG-CoA reductase 1;
DE            EC=1.1.1.34;
GN   Name=HMGR1;
OS   Hevea brasiliensis (Para rubber tree).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Euphorbiaceae; Crotonoideae;
OC   Micrandreae; Hevea.
OX   NCBI_TaxID=3981;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=cv. RRIM 600; TISSUE=Leaf;
RX   MEDLINE=91329691; PubMed=1714317; DOI=10.1007/BF00023422;
RA   Chye M.L., Kush A., Tan C.T., Chua N.H.;
RT   "Characterization of cDNA and genomic clones encoding 3-hydroxy-3-
RT   methylglutaryl-coenzyme A reductase from Hevea brasiliensis.";
RL   Plant Mol. Biol. 16:567-577(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RA   Venkatachalam P., Priya P., Thulaseedharan A.;
RT   "Cloning and nucleotide sequence analysis of HMGR1 gene in Hevea
RT   brasiliensis.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific
CC       precursor of all isoprenoid compounds present in plants.
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein. Mitochondrion membrane; Multi-pass membrane
CC       protein. Plastid membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; X54657; CAA38467.1; -; Genomic_DNA.
DR   EMBL; X54659; CAA38469.1; -; mRNA.
DR   EMBL; AY352338; AAQ63055.1; -; Genomic_DNA.
DR   PIR; S14955; S14955.
DR   HSSP; P04035; 1DQA.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042170; C:plastid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   InterPro; IPR004554; HMG_CoA_Rdtase_I_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 1.
DR   PANTHER; PTHR10572; HMG-CoA_red; 1.
DR   Pfam; PF00368; HMG-CoA_red; 1.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane;
KW   Mitochondrion; NADP; Oxidoreductase; Plastid; Transmembrane.
FT   CHAIN         1    575       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase 1.
FT                                /FTId=PRO_0000114440.
FT   TRANSMEM     29     49       Potential.
FT   TRANSMEM     73     93       Potential.
FT   TRANSMEM    531    551       Potential.
FT   REGION       97    160       Linker (By similarity).
FT   REGION      161    575       Catalytic (By similarity).
FT   ACT_SITE    254    254       Charge relay system (By similarity).
FT   ACT_SITE    386    386       Charge relay system (By similarity).
FT   ACT_SITE    462    462       Charge relay system (By similarity).
FT   ACT_SITE    560    560       Proton donor (By similarity).
FT   CARBOHYD    132    132       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    318    318       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    564    564       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   575 AA;  61695 MW;  BC0A74898B6124D5 CRC64;
     MDTTGRLHHR KHATPVEDRS PTTPKASDAL PLPLYLTNAV FFTLFFSVAY YLLHRWRDKI
     RNSTPLHIVT LSEIVAIVSL IASFIYLLGF FGIDFVQSFI ARASHDVWDL EDTDPNYLID
     EDHRLVTCPP ANISTKTTII AAPTKLPTSE PLIAPLVSEE DEMIVNSVVD GKIPSYSLES
     KLGDCKRAAA IRREALQRMT RRSLEGLPVE GFDYESILGQ CCEMPVGYVQ IPVGIAGPLL
     LNGREYSVPM ATTEGCLVAS TNRGCKAIYL SGGATSVLLK DGMTRAPVVR FASATRAAEL
     KFFLEDPDNF DTLAVVFNKS SRFARLQGIK CSIAGKNLYI RFSCSTGDAM GMNMVSKGVQ
     NVLEFLQSDF SDMDVIGISG NFCSDKKPAA VNWIEGRGKS VVCEAIIKEE VVKKVLKTNV
     ASLVELNMLK NLAGSAVAGA LGGFNAHAGN IVSAIFIATG QDPAQNVESS HCITMMEAVN
     DGKDLHISVT MPSIEVGTVG GGTQLASQSA CLNLLGVKGA NKESPGSNSR LLAAIVAGSV
     LAGELSLMSA IAAGQLVKSH MKYNRSSKDM SKAAS
//