ID   HMDH2_HEVBR             Reviewed;         210 AA.
AC   P29058;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-NOV-2008, entry version 57.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 2;
DE            Short=HMG-CoA reductase 2;
DE            EC=1.1.1.34;
DE   Flags: Fragment;
GN   Name=HMGR2;
OS   Hevea brasiliensis (Para rubber tree).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Euphorbiaceae; Crotonoideae;
OC   Micrandreae; Hevea.
OX   NCBI_TaxID=3981;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. RRIM 600; TISSUE=Leaf;
RX   MEDLINE=91329691; PubMed=1714317; DOI=10.1007/BF00023422;
RA   Chye M.L., Kush A., Tan C.T., Chua N.H.;
RT   "Characterization of cDNA and genomic clones encoding 3-hydroxy-3-
RT   methylglutaryl-coenzyme A reductase from Hevea brasiliensis.";
RL   Plant Mol. Biol. 16:567-577(1991).
CC   -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific
CC       precursor of all isoprenoid compounds present in plants.
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein. Mitochondrion membrane; Multi-pass membrane
CC       protein. Plastid membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; X54658; CAA38468.1; -; mRNA.
DR   PIR; S14953; S14953.
DR   HSSP; P04035; 1DQA.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042170; C:plastid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050662; F:coenzyme binding; IEA:InterPro.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:InterPro.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 1.
DR   PANTHER; PTHR10572; HMG-CoA_red; 1.
DR   Pfam; PF00368; HMG-CoA_red; 1.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; PARTIAL.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane;
KW   Mitochondrion; NADP; Oxidoreductase; Plastid; Transmembrane.
FT   CHAIN        <1    210       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase 2.
FT                                /FTId=PRO_0000114441.
FT   TRANSMEM    166    186       Potential.
FT   ACT_SITE     21     21       Charge relay system (By similarity).
FT   ACT_SITE     97     97       Charge relay system (By similarity).
FT   ACT_SITE    195    195       Proton donor (By similarity).
FT   CARBOHYD    199    199       N-linked (GlcNAc...) (Potential).
FT   NON_TER       1      1
SQ   SEQUENCE   210 AA;  21564 MW;  059C3C30766BD911 CRC64;
     LESDFADMDV IGISGNFCSD KKPAAVNWIE GRGKSVVCEA IIKEEVVKKV LKTDVALLVE
     LNMLKNLAGS AVAGALGGFN AHAGNIVSAI FIATGQDPAQ NVESSHCITM MEAVNDGKDL
     HISVTLPSIE VGTVGGGTQL ASQSACLNLL GVMGACKESP GSYSRLLATI VAGSVLAGEL
     SLMSAIAAGQ LVKSHMKYNR SSKDVSKAAS
//