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UniProtKB/Swiss-Prot entry P29102

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_BRANA
Primary accession number P29102
Secondary accession numbers None
Integrated into Swiss-Prot on December 1, 1992
Sequence was last modified on December 1, 1992 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 57)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase, chloroplastic [Precursor]
Synonyms 3-IPM-DH
IMDH
EC 1.1.1.85
Beta-IPM dehydrogenase
Gene name None
From
Brassica napus (Rape) [TaxID: 3708] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Brassica.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Svalofs Karat 20516-K;
DOI=10.1007/BF00040671; PubMed=1371407 [NCBI, ExPASy, EBI, Israel, Japan]
Ellerstroem M., Josefsson L.G., Rask L., Ronne H.;
"Cloning of a cDNA for rape chloroplast 3-isopropylmalate dehydrogenase by genetic complementation in yeast.";
Plant Mol. Biol. 18:557-566(1992).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X59970; CAA42596.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S20510; S20510.
3D structure databases
HSSP Q56268; 1A05. [HSSP ENTRY / PDB]
ModBase P29102.
Ontologies
GO
GO:0009507; Cellular component: chloroplast (inferred from electronic annotation from UniProtKB-KW).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
ProtoNet P29102.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Chloroplast; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    34  34     Chloroplast (Potential). 
CHAIN   35   406  372     3-isopropylmalate dehydrogenase, chloroplastic. PRO_0000014456
NP_BIND   117   130  14     NAD (By similarity). 
NP_BIND   323   335  13     NAD (By similarity). 
METAL   265   265        Magnesium or manganese (By similarity). 
METAL   289   289        Magnesium or manganese (By similarity). 
METAL   293   293        Magnesium or manganese (By similarity). 
BINDING   137   137        Substrate (By similarity). 
BINDING   147   147        Substrate (By similarity). 
BINDING   175   175        Substrate (By similarity). 
BINDING   265   265        Substrate (By similarity). 
SITE   182   182  1     Important for catalysis (By similarity). 
SITE   233   233  1     Important for catalysis (By similarity). 
Sequence information
Length: 406 AA [This is the length of the unprocessed precursor] Molecular weight: 43351 Da [This is the MW of the unprocessed precursor] CRC64: 2C8CCEA3B28FF192 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAAALQTNIR PVKFPATLRA LTKQSSPAPF RVRCAAASPG KKRYNITLLP GDGIGPEVIS 

        70         80         90        100        110        120 
IAKNVLQQAG SLEGLEFSFQ EMPVGGAALD LVGVPLPEET VSAAKESDAV LLGAIGGYKW 

       130        140        150        160        170        180 
DKNEKHLKPE TGLLQLRAGL KVFANLRPAT VLPQLVDAST LKREVAEGVD LMVVRELTGG 

       190        200        210        220        230        240 
IYFGVPRGIK TNENGEEVGY NTEVYAAHEI DRIARVAFET ARKRRGKLCS VDKANVLDAS 

       250        260        270        280        290        300 
ILWRRRVTAL AAEYPDVELS HMYVDNAAMQ LVRDPKQFDT IVTNNIFGDI LSDEASMITG 

       310        320        330        340        350        360 
SIGMLPSASL SDSGPGLFEP IHGSAPDIAG QDKANPLATI LSAAMLLKYG LGEEKAAKRI 

       370        380        390        400 
EDAVLGALNK GFRTGDIYSA GTKLVGCKEM GEEVLKSVDS HVQASV
P29102 in FASTA format

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