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UniProtKB/Swiss-Prot entry P30234


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHA_MYCTU
Primary accession number P30234
Secondary accession number O33322
Integrated into Swiss-Prot on April 1, 1993
Sequence was last modified on July 15, 1999 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 63)
Name and origin of the protein
Protein name Alanine dehydrogenase
Synonyms EC 1.4.1.1
40 kDa antigen
TB43
Gene name
Name: ald
OrderedLocusNames: Rv2780, MT2850
ORFNames: MTV002.45
From
Mycobacterium tuberculosis [TaxID: 1773] [HAMAP proteome]
Taxonomy Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Corynebacterineae; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 35801 / TMC 107 / Erdman;
PubMed=1587598 [NCBI, ExPASy, EBI, Israel, Japan]
Andersen A.B., Andersen P., Ljungqvist L.;
"Structure and function of a 40,000-molecular-weight protein antigen of Mycobacterium tuberculosis.";
Infect. Immun. 60:2317-2323(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 25618 / H37Rv;
Singh M., Hutter B.;
"Host-vector system for high level expression and purification of enzymatically active L-alanine dehydrogenase of M.tuberculosis in E.coli.";
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 25618 / H37Rv;
DOI=10.1038/31159; PubMed=9634230 [NCBI, ExPASy, EBI, Israel, Japan]
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.;
"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.";
Nature 393:537-544(1998).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=CDC 1551 / Oshkosh;
DOI=10.1128/JB.184.19.5479-5490.2002; PubMed=12218036 [NCBI, ExPASy, EBI, Israel, Japan]
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains.";
J. Bacteriol. 184:5479-5490(2002).
[5]
PROTEIN SEQUENCE OF 1-21.
STRAIN=ATCC 25618 / H37Rv;
PubMed=7822223 [NCBI, ExPASy, EBI, Israel, Japan]
Deshpande R.G., Khan M.B., Bhat D.A., Navalkar R.G.;
"Isolation of a 43 kDa protein from Mycobacterium tuberculosis H37Rv and its identification as a pyridine nucleotide transhydrogenase.";
J. Appl. Bacteriol. 77:639-643(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X63069; CAA44791.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U92472; AAC38804.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BX842580; CAA15575.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000516; AAK47169.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C70883; A43830.
RefSeq NP_217296.1; -.
NP_337355.1; -.
3D structure databases
PDB
2VHV; X-ray; 2.80 A; A/B=1-371.[ExPASy / RCSB / EBI]
2VHW; X-ray; 2.00 A; A/B/C/D/E/F=1-371.[ExPASy / RCSB / EBI]
2VHX; X-ray; 2.00 A; A/B/C/D/E/F=1-371.[ExPASy / RCSB / EBI]
2VHY; X-ray; 2.30 A; A/B=1-371.[ExPASy / RCSB / EBI]
2VHZ; X-ray; 2.04 A; A/B=1-371.[ExPASy / RCSB / EBI]
2VOE; X-ray; 2.60 A; A/B/C/D/E/F=1-371.[ExPASy / RCSB / EBI]
2VOJ; X-ray; 2.60 A; A/C/E=1-371.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2VHV; -.
2VHW; -.
2VHX; -.
2VHY; -.
2VHZ; -.
2VOE; -.
2VOJ; -.
ModBase P30234.
Organism-specific databases
TubercuList Rv2780; -.
Family and domain databases
InterPro IPR007698; Ala_DHase/PNT_C.
IPR008142; Ala_DHase/PNT_CS1.
IPR008143; Ala_DHase/PNT_CS2.
IPR007886; Ala_DHase/PNT_N.
IPR008141; Ala_DHase_PNT.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF01262; AlaDh_PNT_C; 1.
PF05222; AlaDh_PNT_N; 1.
Pfam graphical view of domain structure.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR00518; alaDH; 1.
PROSITE PS00836; ALADH_PNT_1; 1.
PS00837; ALADH_PNT_2; 1.
BLOCKS P30234.
Genome annotation databases
GeneID 888493; -.
925435; -.
GenomeReviews AE000516_GR; MT2850.
AL123456_GR; Rv2780.
KEGG mtc:MT2850; -.
mtu:Rv2780; -.
TIGR MT2850; -.
Phylogenomic databases
HOGENOM P30234; -.
Other
ProtoNet P30234.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Direct protein sequencing; NAD; Oxidoreductase; Secreted.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   371  371     Alanine dehydrogenase. PRO_0000198994
NP_BIND   170   200  31     NAD (By similarity). 
ACT_SITE   96    96        Potential. 
CONFLICT   13    13        E -> EFQ (in Ref. 1; CAA44791). 
CONFLICT   123   123        A -> P (in Ref. 4; AAK47169). 
Sequence information
Length: 371 AA [This is the length of the unprocessed precursor] Molecular weight: 38713 Da [This is the MW of the unprocessed precursor] CRC64: 9DF7540524DC116A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRVGIPTETK NNEFRVAITP AGVAELTRRG HEVLIQAGAG EGSAITDADF KAAGAQLVGT 

        70         80         90        100        110        120 
ADQVWADADL LLKVKEPIAA EYGRLRHGQI LFTFLHLAAS RACTDALLDS GTTSIAYETV 

       130        140        150        160        170        180 
QTADGALPLL APMSEVAGRL AAQVGAYHLM RTQGGRGVLM GGVPGVEPAD VVVIGAGTAG 

       190        200        210        220        230        240 
YNAARIANGM GATVTVLDIN IDKLRQLDAE FCGRIHTRYS SAYELEGAVK RADLVIGAVL 

       250        260        270        280        290        300 
VPGAKAPKLV SNSLVAHMKP GAVLVDIAID QGGCFEGSRP TTYDHPTFAV HDTLFYCVAN 

       310        320        330        340        350        360 
MPASVPKTST YALTNATMPY VLELADHGWR AACRSNPALA KGLSTHEGAL LSERVATDLG 

       370 
VPFTEPASVL A 

P30234 in FASTA format

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