NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
PubMed=8503846 [NCBI, ExPASy, EBI, Israel, Japan]
Leaver M.J., Scott K., George S.G.;
"Cloning and characterization of the major hepatic glutathione S-transferase from a marine teleost flatfish, the plaice (Pleuronectes platessa), with structural similarities to plant, insect and mammalian Theta class isoenzymes.";
Biochem. J. 292:189-195(1993).

Comments

FUNCTION: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
CATALYTIC ACTIVITY: RX + glutathione = HX + R-S-glutathione.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Found in all the tissues examined. Highest values found in liver and in intestinal mucosa.
INDUCTION: By epoxides and by antioxidants.
SIMILARITY: Belongs to the GST superfamily. Theta family.
SIMILARITY: Contains 1 GST C-terminal domain.
SIMILARITY: Contains 1 GST N-terminal domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X63761; CAA45293.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S33308; S33308.

3D structure databases

HSSP

Q9ZVQ3; 1E6B. [HSSP ENTRY / PDB]

ModBase

P30568.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0004364;	Molecular function: glutathione transferase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR010987; Glutathione-S-Trfase_C-like.
IPR004046; GST_C.
IPR004045; GST_N.
IPR012335; Thioredoxin_fold.
Graphical view of domain structure.

Gene3D

G3DSA:1.20.1050.10; GST_C_like; 1.
G3DSA:3.40.30.10; Thioredoxin_fold; 1.

Pfam

PF00043; GST_C; 1.
PF02798; GST_N; 1.
Pfam graphical view of domain structure.

PROSITE

PS50405; GST_CTER; 1.
PS50404; GST_NTER; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P30568.

Phylogenomic databases

HOVERGEN

P30568; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 225`	`225`	`Glutathione S-transferase A.`	`PRO_0000185946`
`DOMAIN`	`3 85`	`83`	`GST N-terminal.`
`DOMAIN`	`92 217`	`126`	`GST C-terminal.`
`ACT_SITE`	`13 13`		`By similarity.`
`BINDING`	`18 18`		`Glutathione (Potential).`
`CONFLICT`	`140 140`		`L -> V (in Ref. 1; figure 2).`
`CONFLICT`	`143 144`		`EL -> DV (in Ref. 1; figure 2).`
`CONFLICT`	`147 148`		`WE -> SQ (in Ref. 1; figure 2).`

Sequence information

Length: 225 AA [This is the length of the unprocessed precursor]

Molecular weight: 25724 Da [This is the MW of the unprocessed precursor]

CRC64: 37899602D07C077F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAKDMTLLWG SGSPPCWRVM IVLEEKNLQA YNSKLLSFEK GEHKSAEVMS MNPRGQLPSF 

        70         80         90        100        110        120 
KHGSKVLNES YAACMYLESQ FKSQGNKLIP DCPAEQAMMY QRMFEGLTLA QKMADVIYYS 

       130        140        150        160        170        180 
WKVPEAERHD SAVKRNKENL STELKLWEEY LQKTSGSFVA GKSFSLADVS VFPGVAYLFR 

       190        200        210        220 
FGLTEERYPQ LTAYYNSLKE RPSIKASWPP TWLESPQGQD MLKDV

P30568 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools