ID   GPX4_NICSY              Reviewed;         169 AA.
AC   P30708;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-NOV-2008, entry version 57.
DE   RecName: Full=Probable phospholipid hydroperoxide glutathione peroxidase;
DE            Short=PHGPx;
DE            EC=1.11.1.12;
DE   AltName: Full=6P229;
OS   Nicotiana sylvestris (Wood tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Protoplast;
RX   MEDLINE=92163033; PubMed=1536938; DOI=10.1007/BF00040684;
RA   Criqui M.-C., Jamet E., Parmentier Y., Marbach J., Durr A., Fleck J.;
RT   "Isolation and characterization of a plant cDNA showing homology to
RT   animal glutathione peroxidases.";
RL   Plant Mol. Biol. 18:623-627(1992).
CC   -!- FUNCTION: Protects cells and enzymes from oxidative damage, by
CC       catalyzing the reduction of hydrogen peroxide, lipid peroxides and
CC       organic hydroperoxide, by glutathione (By similarity).
CC   -!- CATALYTIC ACTIVITY: 2 glutathione + a lipid hydroperoxide =
CC       glutathione disulfide + lipid + 2 H(2)O.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- TISSUE SPECIFICITY: Germinating seed, apex, flower, as well as in
CC       stressed tissues.
CC   -!- SIMILARITY: Belongs to the glutathione peroxidase family.
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DR   EMBL; X60219; CAA42780.1; -; mRNA.
DR   PIR; S20501; S20501.
DR   HSSP; P00435; 1GP1.
DR   PeroxiBase; 2868; NsGPx06.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004602; F:glutathione peroxidase activity; IEA:InterPro.
DR   GO; GO:0047066; F:phospholipid-hydroperoxide glutathione pero...; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006979; P:response to oxidative stress; IEA:InterPro.
DR   InterPro; IPR000889; Glut_peroxidase.
DR   InterPro; IPR001452; SH3.
DR   InterPro; IPR012335; Thioredoxin_fold.
DR   Gene3D; G3DSA:3.40.30.10; Thioredoxin_fold; 1.
DR   PANTHER; PTHR11592; Glut_peroxidase; 1.
DR   Pfam; PF00255; GSHPx; 1.
DR   PIRSF; PIRSF000303; Glutathion_perox; 1.
DR   PRINTS; PR01011; GLUTPROXDASE.
DR   PRINTS; PR00452; SH3DOMAIN.
DR   PROSITE; PS00460; GLUTATHIONE_PEROXID_1; 1.
DR   PROSITE; PS00763; GLUTATHIONE_PEROXID_2; 1.
DR   PROSITE; PS51355; GLUTATHIONE_PEROXID_3; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Oxidoreductase; Peroxidase.
FT   CHAIN         1    169       Probable phospholipid hydroperoxide
FT                                glutathione peroxidase.
FT                                /FTId=PRO_0000066633.
FT   ACT_SITE     43     43       By similarity.
SQ   SEQUENCE   169 AA;  18767 MW;  401919069E1E97C7 CRC64;
     MASQSSKPQS IYDFTVKDAK GNDVDLSIYK GKVLIIVNVA SQCGLTNSNY TDLTEIYKKY
     KDQGLEILAF PCNQFGGQEP GSIEEIQNMV CTRFKAEYPI FDKVDVNGDN AAPLYKFLKS
     SKGGFFGDSI KWNFSKFLVD KEGNVVDRYS PTTTPASMEK DIKKLLGVA
//