ID   AL3A1_BOVIN             Reviewed;         239 AA.
AC   P30907;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-NOV-2008, entry version 62.
DE   RecName: Full=Aldehyde dehydrogenase, dimeric NADP-preferring;
DE            EC=1.2.1.5;
DE   AltName: Full=Aldehyde dehydrogenase family 3 member A1;
DE            Short=Aldehyde dehydrogenase 3;
DE   AltName: Full=Corneal 15.8 kDa protein;
DE   AltName: Full=Corneal protein 54;
DE   AltName: Full=BCP54;
DE   AltName: Full=Transparentin;
DE   Flags: Fragment;
GN   Name=ALDH3A1; Synonyms=ALDH3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Cornea;
RX   MEDLINE=93113316; PubMed=1842923;
RA   Cooper D.L., Baptist E.W.;
RT   "Degenerate oligonucleotide sequence-directed cross-species PCR
RT   cloning of the BCP 54/ALDH 3 cDNA: priming from inverted repeats and
RT   formation of tandem primer arrays.";
RL   PCR Methods Appl. 1:57-62(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-140.
RC   TISSUE=Cornea;
RX   MEDLINE=91200666; PubMed=2016061; DOI=10.1016/0378-1119(91)90174-A;
RA   Cooper D.L., Baptist E.W., Enghild J.J., Isola N.R., Klintworth G.K.;
RT   "Bovine corneal protein 54K (BCP54) is a homologue of the tumor-
RT   associated (class 3) rat aldehyde dehydrogenase (RATALD).";
RL   Gene 98:201-207(1991).
RN   [3]
RP   PARTIAL PROTEIN SEQUENCE.
RX   MEDLINE=91114362; PubMed=2276277;
RA   Cooper D.L., Baptist E.W., Enghild J.J., Lee H., Isola N.R.,
RA   Klintworth G.K.;
RT   "Partial amino acid sequence determination of bovine corneal protein
RT   54 K (BCP 54).";
RL   Curr. Eye Res. 9:781-786(1990).
CC   -!- FUNCTION: This protein may play a central role in corneal
CC       transparency. It represents about 50 percent of the corneal
CC       epithelial soluble proteins. In the cornea, this enzyme may help
CC       in absorbing the damaging UV-B, as well as detoxicating the UV-
CC       induced peroxidic aldehydes.
CC   -!- FUNCTION: This protein preferentially oxidizes aromatic aldehyde
CC       substrates.
CC   -!- CATALYTIC ACTIVITY: An aldehyde + NAD(P)(+) + H(2)O = an acid +
CC       NAD(P)H.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
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DR   EMBL; S51969; AAB24736.2; -; mRNA.
DR   EMBL; M37384; AAA30468.1; -; mRNA.
DR   PIR; PS0412; PS0412.
DR   PIR; T01406; T01406.
DR   UniGene; Bt.13116; -.
DR   HSSP; P11883; 1AD3.
DR   SMR; P30907; 1-230.
DR   Ensembl; ENSBTAG00000021113; Bos taurus.
DR   HOVERGEN; P30907; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004030; F:aldehyde dehydrogenase [NAD(P)+] activity; IEA:InterPro.
DR   GO; GO:0006081; P:cellular aldehyde metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR016163; Ald_DHase_C.
DR   InterPro; IPR016160; Ald_DHase_CS.
DR   InterPro; IPR012394; Ald_DHase_NAD(P).
DR   InterPro; IPR015590; Aldehyde_DHase.
DR   Gene3D; G3DSA:3.40.309.10; Aldehyde_dehydrogenase_C; 1.
DR   PANTHER; PTHR11699; Aldehyde_dehyd; 1.
DR   PANTHER; PTHR11699:SF15; ALDH; 1.
DR   Pfam; PF00171; Aldedh; 2.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; PARTIAL.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; NADP; Oxidoreductase.
FT   CHAIN        <1   >239       Aldehyde dehydrogenase, dimeric NADP-
FT                                preferring.
FT                                /FTId=PRO_0000056469.
FT   ACT_SITE     30     30       By similarity.
FT   NON_TER       1      1
FT   NON_TER     239    239
SQ   SEQUENCE   239 AA;  26743 MW;  16BD07206287716C CRC64;
     NPHYVDKDRD LDIACRRIAW GKFMNSGQTC VAPDYILCDP SIQSQVVEKL KKSLKEFYGE
     DAKKSRDYGR IINSRHFQRV MGLLEGQKVA YGGTGDATTR YIAPTILTDV DPESPVMQEE
     VFGPVLPIMC VRSLEEAIQF ITQREKPLAL YVFSPNDKVI KKMIAETSSG GVTANDVVVH
     ISVHSLPYGG VGDSGMGSYH GRKSFETFSH RRSCLVRPLL NEETLKARYP RARPICPDT
//