ID   DYR_ENTAE               Reviewed;         159 AA.
AC   P31074;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-NOV-2008, entry version 44.
DE   RecName: Full=Dihydrofolate reductase;
DE            EC=1.5.1.3;
GN   Name=folA;
OS   Enterobacter aerogenes (Aerobacter aerogenes).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Enterobacter.
OX   NCBI_TaxID=548;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=92114753; PubMed=1766362;
RA   Garvin L.D., Hardies S.C.;
RT   "Temporal and topological clustering of diverged residues among
RT   enterobacterial dihydrofolate reductases.";
RL   Mol. Biol. Evol. 8:654-668(1991).
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
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DR   EMBL; M26022; AAA24800.1; -; Genomic_DNA.
DR   PIR; A39799; A39799.
DR   HSSP; P00379; 5DFR.
DR   SMR; P31074; 1-159.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR001796; DHFR_reg.
DR   PANTHER; PTHR11549:SF1; DHFR; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   NADP; One-carbon metabolism; Oxidoreductase.
FT   CHAIN         1    159       Dihydrofolate reductase.
FT                                /FTId=PRO_0000186389.
FT   DOMAIN        1    158       DHFR.
SQ   SEQUENCE   159 AA;  17974 MW;  73C9B8D31D0C1C07 CRC64;
     MISLIAALAV DRVIGMENAM PWDLPADLAW FKRNTLNKPV VMGRLTWESI GRPLPGRKNI
     VISSKPGSDD RVQWVKSVDE AIAACGDAEE IMVIGGGRVY EQFLPKAHKL YLTHIDAEVE
     GDTHFPDYDP DEWESVFSEF HDADAQNSHS YCFEILERR
//