ID   AK1D1_RAT               Reviewed;         326 AA.
AC   P31210;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-NOV-2008, entry version 66.
DE   RecName: Full=3-oxo-5-beta-steroid 4-dehydrogenase;
DE            EC=1.3.1.3;
DE   AltName: Full=Delta(4)-3-ketosteroid 5-beta-reductase;
DE   AltName: Full=Delta(4)-3-oxosteroid 5-beta-reductase;
DE   AltName: Full=Aldo-keto reductase family 1 member D1;
GN   Name=Akr1d1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   MEDLINE=91257305; PubMed=1710579; DOI=10.1016/0014-5793(91)80591-P;
RA   Onishi Y., Noshiro M., Shimosato T., Okuda K.;
RT   "Molecular cloning and sequence analysis of cDNA encoding delta 4-3-
RT   ketosteroid 5 beta-reductase of rat liver.";
RL   FEBS Lett. 283:215-218(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Liver;
RX   MEDLINE=92162187; PubMed=1789929;
RA   Onishi Y., Noshiro M., Shimosato T., Okuda K.;
RT   "Delta 4-3-Oxosteroid 5 beta-reductase. Structure and function.";
RL   Biol. Chem. Hoppe-Seyler 372:1039-1049(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   MEDLINE=96133451; PubMed=8550030;
RX   DOI=10.1053/jhep.1996.v23.pm0008550030;
RA   Zhu Y., Fillenwarth M.J., Crabb D., Lumeng L., Lin R.C.;
RT   "Identification of the 37-kd rat liver protein that forms an
RT   acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5 beta-
RT   reductase.";
RL   Hepatology 23:115-122(1996).
CC   -!- FUNCTION: Efficiently catalyzes the reduction of progesterone,
CC       androstenedione, 17-alpha-hydroxyprogesterone and testosterone to
CC       5-beta-reduced metabolites. The bile acid intermediates 7-
CC       alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-
CC       cholesten-3-one can also act as substrates.
CC   -!- CATALYTIC ACTIVITY: 5-beta-cholestan-3-one + NADP(+) = cholest-4-
CC       en-3-one + NADPH.
CC   -!- CATALYTIC ACTIVITY: 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione
CC       + NADP(+) = cortisone.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family.
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DR   EMBL; D17309; BAA04131.1; -; mRNA.
DR   EMBL; S80431; AAB35916.2; -; mRNA.
DR   PIR; S15835; S15835.
DR   RefSeq; NP_620239.1; -.
DR   UniGene; Rn.25716; -.
DR   HSSP; P23457; 1LWI.
DR   PhosphoSite; P31210; -.
DR   Ensembl; ENSRNOG00000013004; Rattus norvegicus.
DR   GeneID; 192242; -.
DR   KEGG; rno:192242; -.
DR   RGD; 620752; Akr1d1.
DR   HOVERGEN; P31210; -.
DR   NextBio; 622866; -.
DR   ArrayExpress; P31210; -.
DR   GermOnline; ENSRNOG00000013004; Rattus norvegicus.
DR   GO; GO:0047568; F:3-oxo-5-beta-steroid 4-dehydrogenase activity; IDA:MGI.
DR   GO; GO:0047787; F:delta4-3-oxosteroid 5beta-reductase activity; IEA:EC.
DR   GO; GO:0030573; P:bile acid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001395; Aldo/ket_red.
DR   Gene3D; G3DSA:3.20.20.100; Aldo/ket_red; 1.
DR   PANTHER; PTHR11732; Aldo/ket_red; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   ProDom; PD000288; Aldo/ket_red; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   1: Evidence at protein level;
KW   Bile acid catabolism; Cytoplasm; Direct protein sequencing;
KW   Lipid metabolism; NADP; Oxidoreductase; Steroid metabolism.
FT   CHAIN         1    326       3-oxo-5-beta-steroid 4-dehydrogenase.
FT                                /FTId=PRO_0000124672.
FT   NP_BIND     168    169       NADP (By similarity).
FT   NP_BIND     220    224       NADP (By similarity).
FT   NP_BIND     273    283       NADP (By similarity).
FT   ACT_SITE     57     57       Proton donor (By similarity).
FT   BINDING      52     52       NADP (By similarity).
FT   BINDING     192    192       NADP (By similarity).
FT   BINDING     230    230       Substrate (By similarity).
SQ   SEQUENCE   326 AA;  37378 MW;  32D8266BFA62CE99 CRC64;
     MNLSTANHHI PLNDGNSIPI IGLGTYSDPR PVPGKTFIAV KTAIDEGYRH IDGAYVYRNE
     HEVGEAIREK VAEGKVKREE IFYCGKLWST DHDPEMVRPA LERTLQTLKL DYIDLYIIEM
     PMAFKPGEEF YPKDENGRVI YHKSNLCATW EALEACKDAG LVKSLGVSNF NRRQLEVILN
     KPGLKYKPVT NQVECHPYFT QTKLLEVSAS SMTSFIVAYS PLGTCRNPLW VNVSSPPLLK
     DELLTSLGKK YNKTQAQIVL RFDIQRGLVV IPKSTTPERI KENFQIFDFS LTKEEMKDIE
     ALNKNVRFVE MLMWSDHPEY PFHDEY
//