[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 37-52. PubMed=1587288 [NCBI, ExPASy, EBI, Israel, Japan] Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A.; "The quinone-reactive Ni/Fe-hydrogenase of Wolinella succinogenes."; Eur. J. Biochem. 206:93-102(1992).
[2]	ERRATUM. PubMed=8319698 [NCBI, ExPASy, EBI, Israel, Japan] Dross F., Geisler V., Lenger R., Theis F., Krafft T., Fahrenholz F., Kojro E., Duchene A., Tripier D., Juvenal K., Kroeger A.; Eur. J. Biochem. 214:949-950(1993).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=DSMZ 1740; DOI=10.1073/pnas.1932838100; PubMed=14500908 [NCBI, ExPASy, EBI, Israel, Japan] Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O., Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B., Meyer F., Lederer H., Schuster S.C.; "Complete genome sequence and analysis of Wolinella succinogenes."; Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003).

Comments

CATALYTIC ACTIVITY: H₂ + menaquinone = menaquinol.
COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X65189; CAA46302.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BX571661; CAE10714.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S33852; S33852.

RefSeq

NP_907814.1; -.

3D structure databases

HSSP

P18187; 1FRF. [HSSP ENTRY / PDB]

ModBase

P31884.

Enzyme and pathway databases

BioCyc

WSUC273121:WS1687-MON; -.

Family and domain databases

InterPro

IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006137; OxRdtase_q6.
IPR006311; Tat.
Graphical view of domain structure.

Pfam

PF08425; NiFe_dehyd_N; 1.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000310; NiFe_hyd_ssu; 1.

PRINTS

PR00614; NIHGNASESMLL.

TIGRFAMs

TIGR00391; hydA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P31884.

Genome annotation databases

2554748; -.

BX571656_GR; WS1687.

wsu:WS1687; -.

fig|273121.1.peg.1541; -.

Phylogenomic databases

HOGENOM

P31884; -.

Genome annotation databases

CMR

P31884; WS1687.

Other

ProtoNet

P31884.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3Fe-4S; 4Fe-4S; Cell membrane; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 36`	`36`	`Tat-type signal.`
`CHAIN`	`37 354`	`318`	`Quinone-reactive Ni/Fe-hydrogenase small chain.`	`PRO_0000013437`
`METAL`	`53 53`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`56 56`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`153 153`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`186 186`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`224 224`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`227 227`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`252 252`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`258 258`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`267 267`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`286 286`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`289 289`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`

Sequence information

Length: 354 AA [This is the length of the unprocessed precursor]

Molecular weight: 38294 Da [This is the MW of the unprocessed precursor]

CRC64: C39CC21D2F5AB7D0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLEEKGIERR DFMKWAGAMT AMLSLPATFT PLTAKAAELA DRLPVIWLHM AECTGCSESL 

        70         80         90        100        110        120 
LRTDGPGIDS LIFDYISLEY HETVMAAAGW QAEHNLEHAI EKYKGRYVLM VEGGIPAGSS 

       130        140        150        160        170        180 
EFYLTVGPHG TTGAEHARHA SANAAAIFAI GSCSSFGGVQ AARPNPTNAQ PLSKVTNKPV 

       190        200        210        220        230        240 
INVPGCPPSE KNIVGNVLHF ILFGTLPSVD AFNRPMWAYG LRIHDLCERR GRFDAGEFVQ 

       250        260        270        280        290        300 
EFGDEGAKKG YCLYKVGCKG PYTFNNCSKL RFNQHTSWPV QAGHGCIGCS EPDFWDTMGP 

       310        320        330        340        350 
FEEPVANRLY ATAFDGLGAD KTADKIGITL LAATAVGVAA HAVLSMMVKD KENN

P31884 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools