ID   MBHL_RALEH              Reviewed;         618 AA.
AC   P31891; Q7WXU5;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   25-NOV-2008, entry version 63.
DE   RecName: Full=Uptake hydrogenase large subunit;
DE            EC=1.12.99.6;
DE   AltName: Full=Hydrogenlyase;
DE   AltName: Full=Membrane-bound hydrogenase large subunit;
GN   Name=hoxG; OrderedLocusNames=PHG002;
OS   Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
OS   (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier
OS   337)).
OG   Plasmid megaplasmid pHG1.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93015670; PubMed=1383192;
RA   Kortlueke C., Horstmann K., Schwartz E., Rohde M., Binsack R.,
RA   Friedrich B.;
RT   "A gene complex coding for the membrane-bound hydrogenase of
RT   Alcaligenes eutrophus H16.";
RL   J. Bacteriol. 174:6277-6289(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22830147; PubMed=12948488; DOI=10.1016/S0022-2836(03)00894-5;
RA   Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B.,
RA   Gottschalk G.;
RT   "Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16
RT   megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and
RT   anaerobiosis.";
RL   J. Mol. Biol. 332:369-383(2003).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-31.
RX   MEDLINE=89166625; PubMed=2493816; DOI=10.1016/0167-4838(89)90225-2;
RA   Lorenz B., Schneider K., Kratzin H., Schlegel H.G.;
RT   "Immunological comparison of subunits isolated from various
RT   hydrogenases of aerobic hydrogen bacteria.";
RL   Biochim. Biophys. Acta 995:1-9(1989).
CC   -!- FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to
CC       increase the production of ATP and to protect nitrogenase against
CC       inhibition or damage by O(2) under carbon- or phosphate-limited
CC       conditions.
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 nickel ion per subunit (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large
CC       subunit family.
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DR   EMBL; M96433; AAA16462.1; -; Unassigned_DNA.
DR   EMBL; AY305378; AAP85758.1; -; Genomic_DNA.
DR   PIR; B43255; B43255.
DR   RefSeq; NP_942644.1; -.
DR   HSSP; P21852; 1UBL.
DR   GeneID; 2656805; -.
DR   GenomeReviews; AY305378_GR; PHG002.
DR   KEGG; reh:PHG002; -.
DR   HOGENOM; P31891; -.
DR   BioCyc; MetaCyc:HOXGALCA-MON; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0016151; F:nickel ion binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Complete proteome; Direct protein sequencing; Membrane;
KW   Metal-binding; Nickel; Oxidoreductase; Plasmid.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    618       Uptake hydrogenase large subunit.
FT                                /FTId=PRO_0000199706.
FT   METAL        75     75       Nickel (Potential).
FT   METAL        78     78       Nickel (Potential).
FT   METAL       597    597       Nickel (Potential).
FT   METAL       600    600       Nickel (Potential).
FT   CONFLICT    578    578       M -> V (in Ref. 1; AAA16462).
SQ   SEQUENCE   618 AA;  68795 MW;  8899BA7486865D3F CRC64;
     MSAYATQGFN LDDRGRRIVV DPVTRIEGHM RCEVNVDANN VIRNAVSTGT MWRGLEVILK
     GRDPRDAWAF VERICGVCTG CHALASVRAV ENALDIRIPK NAHLIREIMA KTLQVHDHAV
     HFYHLHALDW VDVMSALKAD PKRTSELQQL VSPAHPLSSA GYFRDIQNRL KRFVESGQLG
     PFMNGYWGSK AYVLPPEANL MAVTHYLEAL DLQKEWVKIH TIFGGKNPHP NYLVGGVPCA
     INLDGIGAAS APVNMERLSF VKARIDEIIE FNKNVYVPDV LAIGTLYKQA GWLYGGGLAA
     TNVLDYGEYP NVAYNKSTDQ LPGGAILNGN WDEVFPVDPR DSQQVQEFVS HSWYKYADES
     VGLHPWDGVT EPNYVLGANT KGTRTRIEQI DESAKYSWIK SPRWRGHAME VGPLSRYILA
     YAHARSGNKY AERPKEQLEY SAQMINSAIP KALGLPETQY TLKQLLPSTI GRTLARALES
     QYCGEMMHSD WHDLVANIRA GDTATANVDK WDPATWPLQA KGVGTVAAPR GALGHWIRIK
     DGRIENYQCV VPTTWNGSPR DYKGQIGAFE ASLMNTPMVN PEQPVEILRT LHSFDPCLAC
     STHVMSAEGQ ELTTVKVR
//