[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=1383192 [NCBI, ExPASy, EBI, Israel, Japan] Kortlueke C., Horstmann K., Schwartz E., Rohde M., Binsack R., Friedrich B.; "A gene complex coding for the membrane-bound hydrogenase of Alcaligenes eutrophus H16."; J. Bacteriol. 174:6277-6289(1992).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1016/S0022-2836(03)00894-5; PubMed=12948488 [NCBI, ExPASy, EBI, Israel, Japan] Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B., Gottschalk G.; "Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16 megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and anaerobiosis."; J. Mol. Biol. 332:369-383(2003).
[3]	PROTEIN SEQUENCE OF 44-59. DOI=10.1016/0167-4838(89)90225-2; PubMed=2493816 [NCBI, ExPASy, EBI, Israel, Japan] Lorenz B., Schneider K., Kratzin H., Schlegel H.G.; "Immunological comparison of subunits isolated from various hydrogenases of aerobic hydrogen bacteria."; Biochim. Biophys. Acta 995:1-9(1989).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M96433; AAA16461.1; -; Unassigned_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY305378; AAP85757.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A43255; A43255.

RefSeq

NP_942643.1; -.

3D structure databases

HSSP

P18187; 1FRF. [HSSP ENTRY / PDB]

ModBase

P31892.

Enzyme and pathway databases

BioCyc

MetaCyc:HOXKALCA-MON; -.

Family and domain databases

InterPro

IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006137; OxRdtase_q6.
IPR006311; Tat.
Graphical view of domain structure.

Pfam

PF08425; NiFe_dehyd_N; 1.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000310; NiFe_hyd_ssu; 1.

PRINTS

PR00614; NIHGNASESMLL.

TIGRFAMs

TIGR00391; hydA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P31892.

Genome annotation databases

GeneID

2656804; -.

GenomeReviews

AY305378_GR; PHG001.

KEGG

reh:PHG001; -.

Phylogenomic databases

HOGENOM

P31892; -.

Genome annotation databases

CMR

P31892; PHG001.

Other

ProtoNet

P31892.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3Fe-4S; 4Fe-4S; Cell membrane; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding; Oxidoreductase; Plasmid; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 43`	`43`	`Tat-type signal.`
`CHAIN`	`44 360`	`317`	`Uptake hydrogenase small subunit.`	`PRO_0000013421`
`METAL`	`60 60`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`63 63`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`158 158`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`192 192`		`Iron-sulfur 1 (4Fe-4S) (By similarity).`
`METAL`	`230 230`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen (By similarity).`
`METAL`	`233 233`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`258 258`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`264 264`		`Iron-sulfur 2 (4Fe-4S) (By similarity).`
`METAL`	`273 273`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`292 292`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`
`METAL`	`295 295`		`Iron-sulfur 3 (3Fe-4S) (By similarity).`

Sequence information

Length: 360 AA [This is the length of the unprocessed precursor]

Molecular weight: 39472 Da [This is the MW of the unprocessed precursor]

CRC64: D2BB15D85902AF0C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVETFYEVMR RQGISRRSFL KYCSLTATSL GLGPSFLPQI AHAMETKPRT PVLWLHGLEC 

        70         80         90        100        110        120 
TCCSESFIRS AHPLAKDVVL SMISLDYDDT LMAAAGHQAE AILEEIMTKY KGNYILAVEG 

       130        140        150        160        170        180 
NPPLNQDGMS CIIGGRPFIE QLKYVAKDAK AIISWGSCAS WGCVQAAKPN PTQATPVHKV 

       190        200        210        220        230        240 
ITDKPIIKVP GCPPIAEVMT GVITYMLTFD RIPELDRQGR PKMFYSQRIH DKCYRRPHFD 

       250        260        270        280        290        300 
AGQFVEEWDD ESARKGFCLY KMGCKGPTTY NACSTTRWNE GTSFPIQSGH GCIGCSEDGF 

       310        320        330        340        350        360 
WDKGSFYDRL TGISQFGVEA NADKIGGTAS VVVGAAVTAH AAASAIKRAS KKNETSGSEH

P31892 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools