ID   MBHS_RALEH              Reviewed;         360 AA.
AC   P31892;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-NOV-2008, entry version 73.
DE   RecName: Full=Uptake hydrogenase small subunit;
DE            EC=1.12.99.6;
DE   AltName: Full=Hydrogenlyase;
DE   AltName: Full=Membrane-bound hydrogenase small subunit;
DE   Flags: Precursor;
GN   Name=hoxK; OrderedLocusNames=PHG001;
OS   Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)
OS   (Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier
OS   337)).
OG   Plasmid megaplasmid pHG1.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93015670; PubMed=1383192;
RA   Kortlueke C., Horstmann K., Schwartz E., Rohde M., Binsack R.,
RA   Friedrich B.;
RT   "A gene complex coding for the membrane-bound hydrogenase of
RT   Alcaligenes eutrophus H16.";
RL   J. Bacteriol. 174:6277-6289(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22830147; PubMed=12948488; DOI=10.1016/S0022-2836(03)00894-5;
RA   Schwartz E., Henne A., Cramm R., Eitinger T., Friedrich B.,
RA   Gottschalk G.;
RT   "Complete nucleotide sequence of pHG1: a Ralstonia eutropha H16
RT   megaplasmid encoding key enzymes of H(2)-based lithoautotrophy and
RT   anaerobiosis.";
RL   J. Mol. Biol. 332:369-383(2003).
RN   [3]
RP   PROTEIN SEQUENCE OF 44-59.
RX   MEDLINE=89166625; PubMed=2493816; DOI=10.1016/0167-4838(89)90225-2;
RA   Lorenz B., Schneider K., Kratzin H., Schlegel H.G.;
RT   "Immunological comparison of subunits isolated from various
RT   hydrogenases of aerobic hydrogen bacteria.";
RL   Biochim. Biophys. Acta 995:1-9(1989).
CC   -!- FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to
CC       increase the production of ATP and to protect nitrogenase against
CC       inhibition or damage by O(2) under carbon- or phosphate-limited
CC       conditions.
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has been experimentally proven.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small
CC       subunit family.
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DR   EMBL; M96433; AAA16461.1; -; Unassigned_DNA.
DR   EMBL; AY305378; AAP85757.1; -; Genomic_DNA.
DR   PIR; A43255; A43255.
DR   RefSeq; NP_942643.1; -.
DR   HSSP; P18187; 1FRF.
DR   GeneID; 2656804; -.
DR   GenomeReviews; AY305378_GR; PHG001.
DR   KEGG; reh:PHG001; -.
DR   HOGENOM; P31892; -.
DR   BioCyc; MetaCyc:HOXKALCA-MON; -.
DR   GO; GO:0009375; C:ferredoxin hydrogenase complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to u...; IEA:InterPro.
DR   InterPro; IPR001821; NiFe_hyd_ssu.
DR   InterPro; IPR013634; NiFe_hyd_ssu_N.
DR   InterPro; IPR006137; OxRdtase_q6.
DR   InterPro; IPR006311; Tat.
DR   Pfam; PF08425; NiFe_dehyd_N; 1.
DR   Pfam; PF01058; Oxidored_q6; 1.
DR   PIRSF; PIRSF000310; NiFe_hyd_ssu; 1.
DR   PRINTS; PR00614; NIHGNASESMLL.
DR   TIGRFAMs; TIGR00391; hydA; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   1: Evidence at protein level;
KW   3Fe-4S; 4Fe-4S; Cell membrane; Complete proteome;
KW   Direct protein sequencing; Iron; Iron-sulfur; Membrane; Metal-binding;
KW   Oxidoreductase; Plasmid; Signal.
FT   SIGNAL        1     43       Tat-type signal.
FT   CHAIN        44    360       Uptake hydrogenase small subunit.
FT                                /FTId=PRO_0000013421.
FT   METAL        60     60       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL        63     63       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       158    158       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       192    192       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       230    230       Iron-sulfur 2 (4Fe-4S); via pros nitrogen
FT                                (By similarity).
FT   METAL       233    233       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       258    258       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       264    264       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       273    273       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       292    292       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       295    295       Iron-sulfur 3 (3Fe-4S) (By similarity).
SQ   SEQUENCE   360 AA;  39472 MW;  D2BB15D85902AF0C CRC64;
     MVETFYEVMR RQGISRRSFL KYCSLTATSL GLGPSFLPQI AHAMETKPRT PVLWLHGLEC
     TCCSESFIRS AHPLAKDVVL SMISLDYDDT LMAAAGHQAE AILEEIMTKY KGNYILAVEG
     NPPLNQDGMS CIIGGRPFIE QLKYVAKDAK AIISWGSCAS WGCVQAAKPN PTQATPVHKV
     ITDKPIIKVP GCPPIAEVMT GVITYMLTFD RIPELDRQGR PKMFYSQRIH DKCYRRPHFD
     AGQFVEEWDD ESARKGFCLY KMGCKGPTTY NACSTTRWNE GTSFPIQSGH GCIGCSEDGF
     WDKGSFYDRL TGISQFGVEA NADKIGGTAS VVVGAAVTAH AAASAIKRAS KKNETSGSEH
//