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UniProtKB/Swiss-Prot entry P32322

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name P5CR1_HUMAN
Primary accession number P32322
Secondary accession numbers Q6FHI4 Q96DI6
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on March 1, 2005 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 72)
Name and origin of the protein
Protein name Pyrroline-5-carboxylate reductase 1, mitochondrial [Precursor]
Synonyms P5C reductase 1
P5CR 1
EC 1.5.1.2
Gene name
Name: PYCR1
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=1730675 [NCBI, ExPASy, EBI, Israel, Japan]
Dougherty K.M., Brandriss M.C., Valle D.;
"Cloning human pyrroline-5-carboxylate reductase cDNA by complementation in Saccharomyces cerevisiae.";
J. Biol. Chem. 267:871-875(1992).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.;
"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).";
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Lymph, and Placenta;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
 X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS), AND SUBUNIT.
DOI=10.1016/j.jmb.2006.04.053; PubMed=16730026 [NCBI, ExPASy, EBI, Israel, Japan]
Meng Z., Lou Z., Liu Z., Li M., Zhao X., Bartlam M., Rao Z.;
"Crystal structure of human pyrroline-5-carboxylate reductase.";
J. Mol. Biol. 359:1364-1377(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M77836; AAA36407.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CR541769; CAG46568.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC001504; AAH01504.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC071842; AAH71842.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A41770; A41770.
RefSeq NP_008838.2; -.
UniGene Hs.458332
3D structure databases
PDB
2GER; X-ray; 3.10 A; A/B/C/D/E=1-319.[ExPASy / RCSB / EBI]
2GR9; X-ray; 3.10 A; A/B/C/D/E=1-275.[ExPASy / RCSB / EBI]
2GRA; X-ray; 3.10 A; A/B/C/D/E=1-275.[ExPASy / RCSB / EBI]
2IZZ; X-ray; 1.95 A; A/B/C/D/E=1-300.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2GER; -.
2GR9; -.
2GRA; -.
2IZZ; -.
ModBase P32322.
Enzyme and pathway databases
BioCyc MetaCyc:MON-11444; -.
Reactome REACT_13; Metabolism of amino acids.
Organism-specific databases
H-InvDB HIX0014258; -.
HGNC HGNC:9721; PYCR1.
GenAtlas PYCR1.
MIM 179035; gene. [NCBI / EBI]
PharmGKB PA34064; -.
GeneCards P32322.
Gene expression databases
ArrayExpress P32322; -.
CleanEx HS_PYCR1; -.
GermOnline ENSG00000183010; Homo sapiens.
Ontologies
GO
GO:0004735; Molecular function: pyrroline-5-carboxylate reductase activity (traceable author statement from ProtInc).
GO:0006561; Biological process: proline biosynthetic process (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR016040; NAD(P)-bd.
IPR004455; NADP_OxRdtase_F420.
IPR000304; P5CR.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11645; P5CR; 1.
Pfam PF03807; F420_oxidored; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000193; Pyrrol-5-carb_rd; 1.
TIGRFAMs TIGR00112; proC; 1.
PROSITE PS00521; P5CR; 1.
BLOCKS P32322.
Genome annotation databases
Ensembl ENSG00000183010; Homo sapiens. [Contig view]
GeneID 5831; -.
KEGG hsa:5831; -.
Phylogenomic databases
HOGENOM P32322; -.
HOVERGEN P32322; -.
Other
DrugBank DB00172; L-Proline.
DB00157; NADH.
SOURCE PYCR1; Homo sapiens.
ProtoNet P32322.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Amino-acid biosynthesis; Mitochondrion; NADP; Oxidoreductase; Proline biosynthesis; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    26  26     Mitochondrion (Potential). 
CHAIN   27   319  293     Pyrroline-5-carboxylate reductase 1, mitochondrial. PRO_0000187314
CONFLICT   155   155        S -> T (in Ref. 1; AAA36407). 
CONFLICT   317   317        G -> S (in Ref. 2; CAG46568). 
STRAND   3     7  5      
HELIX   10    21  12      
HELIX   27    29  3      
STRAND   30    33  4      
HELIX   40    48  9      
STRAND   51    54  4      
HELIX   56    62  7      
STRAND   64    68  5      
HELIX   72    74  3      
HELIX   75    82  8      
HELIX   83    85  3      
STRAND   91    94  4      
HELIX   101   109  9      
STRAND   116   121  6      
HELIX   124   128  5      
STRAND   131   137  7      
HELIX   143   154  12      
STRAND   157   161  5      
HELIX   164   166  3      
HELIX   167   173  7      
TURN   174   176  3      
HELIX   177   194  18      
HELIX   199   219  21      
HELIX   224   231  8      
HELIX   237   247  11      
HELIX   250   270  21      
Sequence information
Length: 319 AA [This is the length of the unprocessed precursor] Molecular weight: 33361 Da [This is the MW of the unprocessed precursor] CRC64: 9E8C4DED0638EFC5 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV 

        70         80         90        100        110        120 
QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC 

       130        140        150        160        170        180 
MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY 

       190        200        210        220        230        240 
AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH 

       250        260        270        280        290        300 
ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL 

       310 
SPSGHTKLLP RSLAPAGKD
P32322 in FASTA format

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