ID   NDI1_YEAST              Reviewed;         513 AA.
AC   P32340;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-NOV-2008, entry version 87.
DE   RecName: Full=Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial;
DE            EC=1.6.5.3;
DE   AltName: Full=Internal NADH dehydrogenase;
DE   Flags: Precursor;
GN   Name=NDI1; OrderedLocusNames=YML120C; ORFNames=YM7056.06C;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 27-36 AND
RP   41-50.
RC   STRAIN=YP102;
RX   MEDLINE=92137248; PubMed=1735444;
RA   de Vries S., van Witzenburg R., Grivell L.A., Marres C.A.M.;
RT   "Primary structure and import pathway of the rotenone-insensitive
RT   NADH-ubiquinone oxidoreductase of mitochondria from Saccharomyces
RT   cerevisiae.";
RL   Eur. J. Biochem. 203:587-592(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=97313268; PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S.,
RA   Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A.,
RA   Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome
RT   XIII.";
RL   Nature 387:90-93(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   MEDLINE=21393706; PubMed=11502169; DOI=10.1021/bi010277r;
RA   Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N.,
RA   Manon S., Schmitter J.-M.;
RT   "Yeast mitochondrial dehydrogenases are associated in a supramolecular
RT   complex.";
RL   Biochemistry 40:9758-9769(2001).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27 AND THR-28, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17761666; DOI=10.1074/mcp.M700098-MCP200;
RA   Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B.,
RA   van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.;
RT   "Profiling phosphoproteins of yeast mitochondria reveals a role of
RT   phosphorylation in assembly of the ATP synthase.";
RL   Mol. Cell. Proteomics 6:1896-1906(2007).
CC   -!- FUNCTION: Catalyzes the oxidation of NADH generated inside the
CC       Mitochondrion.
CC   -!- CATALYTIC ACTIVITY: NADH + ubiquinone = NAD(+) + ubiquinol.
CC   -!- COFACTOR: Binds 1 FAD per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral
CC       membrane protein; Matrix side. Note=Bound to the mitochondrial
CC       inner membrane facing the matrix site.
CC   -!- MISCELLANEOUS: Present with 5240 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the NADH dehydrogenase family.
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DR   EMBL; X61590; CAA43787.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; Z49218; CAA89160.1; -; Genomic_DNA.
DR   EMBL; AY723851; AAU09768.1; -; Genomic_DNA.
DR   PIR; S26704; S26704.
DR   RefSeq; NP_013586.1; -.
DR   DIP; DIP:5554N; -.
DR   IntAct; P32340; -.
DR   PeptideAtlas; P32340; -.
DR   Ensembl; YML120C; Saccharomyces cerevisiae.
DR   GeneID; 854919; -.
DR   GenomeReviews; Z71257_GR; YML120C.
DR   KEGG; sce:YML120C; -.
DR   NMPDR; fig|4932.3.peg.4622; -.
DR   CYGD; YML120c; -.
DR   SGD; S000004589; NDI1.
DR   HOGENOM; P32340; -.
DR   LinkHub; P32340; -.
DR   NextBio; 977929; -.
DR   ArrayExpress; P32340; -.
DR   GermOnline; YML120C; Saccharomyces cerevisiae.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005759; C:mitochondrial matrix; IDA:SGD.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:EC.
DR   GO; GO:0001300; P:chronological cell aging; IMP:SGD.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to u...; IDA:SGD.
DR   GO; GO:0006116; P:NADH oxidation; IDA:SGD.
DR   GO; GO:0043065; P:positive regulation of apoptosis; IMP:SGD.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR001327; Pyr_OxRdtase_NAD_bd.
DR   Pfam; PF00070; Pyr_redox; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PRINTS; PR00368; FADPNR.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; FAD; Flavoprotein;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; NAD;
KW   Oxidoreductase; Phosphoprotein; Transit peptide; Ubiquinone.
FT   TRANSIT       1     26       Mitochondrion.
FT   CHAIN        27    513       Rotenone-insensitive NADH-ubiquinone
FT                                oxidoreductase, mitochondrial.
FT                                /FTId=PRO_0000021793.
FT   NP_BIND      55     85       FAD (By similarity).
FT   NP_BIND     229    265       NAD (By similarity).
FT   MOD_RES      27     27       Phosphoserine.
FT   MOD_RES      28     28       Phosphothreonine.
FT   VARIANT      10     10       K -> R.
SQ   SEQUENCE   513 AA;  57250 MW;  E3A43D75A1ADCF3B CRC64;
     MLSKNLYSNK RLLTSTNTLV RFASTRSTGV ENSGAGPTSF KTMKVIDPQH SDKPNVLILG
     SGWGAISFLK HIDTKKYNVS IISPRSYFLF TPLLPSAPVG TVDEKSIIEP IVNFALKKKG
     NVTYYEAEAT SINPDRNTVT IKSLSAVSQL YQPENHLGLH QAEPAEIKYD YLISAVGAEP
     NTFGIPGVTD YGHFLKEIPN SLEIRRTFAA NLEKANLLPK GDPERRRLLS IVVVGGGPTG
     VEAAGELQDY VHQDLRKFLP ALAEEVQIHL VEALPIVLNM FEKKLSSYAQ SHLENTSIKV
     HLRTAVAKVE EKQLLAKTKH EDGKITEETI PYGTLIWATG NKARPVITDL FKKIPEQNSS
     KRGLAVNDFL QVKGSNNIFA IGDNAFAGLP PTAQVAHQEA EYLAKNFDKM AQIPNFQKNL
     SSRKDKIDLL FEENNFKPFK YNDLGALAYL GSERAIATIR SGKRTFYTGG GLMTFYLWRI
     LYLSMILSAR SRLKVFFDWI KLAFFKRDFF KGL
//