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UniProtKB/Swiss-Prot entry P32462


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ERG24_YEAST
Primary accession number P32462
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1993 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 62)
Name and origin of the protein
Protein name Delta(14)-sterol reductase
Synonyms EC 1.3.1.70
C-14 sterol reductase
Sterol C14-reductase
Gene name
Name: ERG24
OrderedLocusNames: YNL280C
ORFNames: N0593
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=1418625 [NCBI, ExPASy, EBI, Israel, Japan]
Lorenz R.T., Parks L.W.;
"Cloning, sequencing, and disruption of the gene encoding sterol C-14 reductase in Saccharomyces cerevisiae.";
DNA Cell Biol. 11:685-692(1992).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(94)90728-5; PubMed=8125337 [NCBI, ExPASy, EBI, Israel, Japan]
Lai M.H., Bard M., Pierson C.A., Alexander J.F., Goebl M., Carter G.T., Kirsch D.R.;
"The identification of a gene family in the Saccharomyces cerevisiae ergosterol biosynthesis pathway.";
Gene 140:41-49(1994).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169873 [NCBI, ExPASy, EBI, Israel, Japan]
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.";
Nature 387:93-98(1997).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[5]
TOPOLOGY [LARGE SCALE ANALYSIS].
DOI=10.1073/pnas.0604075103; PubMed=16847258 [NCBI, ExPASy, EBI, Israel, Japan]
Kim H., Melen K., Oesterberg M., von Heijne G.;
"A global topology map of the Saccharomyces cerevisiae membrane proteome.";
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M99419; AAA18256.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S69420; AAB30203.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z71556; CAA96192.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S30769; S30769.
RefSeq NP_014119.1; -.
3D structure databases
ModBase P32462.
Protein-protein interaction databases
DIP DIP:5155N; -.
IntAct P32462; -.
Enzyme and pathway databases
BioCyc MetaCyc:YNL280C-MON; -.
Organism-specific databases
CYGD YNL280c; -.
SGD S000005224; ERG24.
Yeast-GFP YNL280C.
Gene expression databases
ArrayExpress P32462; -.
GermOnline YNL280C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0050613; Molecular function: delta14-sterol reductase activity (inferred from electronic annotation from EC).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0016126; Biological process: sterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001171; ERG4_ERG24.
Graphical view of domain structure.
Pfam PF01222; ERG4_ERG24; 1.
Pfam graphical view of domain structure.
PROSITE PS01017; STEROL_REDUCT_1; 1.
PS01018; STEROL_REDUCT_2; 1.
ProtoNet P32462.
Proteomic databases
PeptideAtlas P32462; -.
Genome annotation databases
Ensembl YNL280C; Saccharomyces cerevisiae. [Contig view]
GeneID 855441; -.
GenomeReviews Y13139_GR; YNL280C.
KEGG sce:YNL280C; -.
NMPDR fig|4932.3.peg.5183; -.
Phylogenomic databases
HOGENOM P32462; -.
Other
LinkHub P32462; -.
NextBio 979334; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   438  438     Delta(14)-sterol reductase. PRO_0000207494
TOPO_DOM   1    13  13     Lumenal (Potential). 
TRANSMEM   14    34  21     Potential. 
TOPO_DOM   35    71  37     Cytoplasmic (Potential). 
TRANSMEM   72    90  19     Potential. 
TOPO_DOM   91   109  19     Lumenal (Potential). 
TRANSMEM   110   127  18     Potential. 
TOPO_DOM   128   147  20     Cytoplasmic (Potential). 
TRANSMEM   148   172  25     Potential. 
TOPO_DOM   173   242  70     Lumenal (Potential). 
TRANSMEM   243   263  21     Potential. 
TOPO_DOM   264   308  45     Cytoplasmic (Potential). 
TRANSMEM   309   328  20     Potential. 
TOPO_DOM   329   368  40     Lumenal (Potential). 
TRANSMEM   369   387  19     Potential. 
TOPO_DOM   388   438  51     Cytoplasmic (Potential). 
CONFLICT   253   253        L -> S (in Ref. 2; AAB30203). 
Sequence information
Length: 438 AA [This is the length of the unprocessed precursor] Molecular weight: 50615 Da [This is the MW of the unprocessed precursor] CRC64: B9368B004506C0F3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVSALNPRTT EFEFGGLIGA LGISIGLPVF TIILNQMIRP DYFIKGFFQN FDIVELWNGI 

        70         80         90        100        110        120 
KPLRYYLGNR ELWTVYCLWY GILAVLDVIL PGRVMKGVQL RDGSKLSYKI NGIAMSTTLV 

       130        140        150        160        170        180 
LVLAIRWKLT DGQLPELQYL YENHVSLCII SILFSFFLAT YCYVASFIPL IFKKNGNGKR 

       190        200        210        220        230        240 
EKILALGGNS GNIIYDWFIG RELNPRLGPL DIKMFSELRP GMLLWLLINL SCLHHHYLKT 

       250        260        270        280        290        300 
GKINDALVLV NFLQGFYIFD GVLNEEGVLT MMDITTDGFG FMLAFGDLSL VPFTYSLQAR 

       310        320        330        340        350        360 
YLSVSPVELG WVKVVGILAI MFLGFHIFHS ANKQKSEFRQ GKLENLKSIQ TKRGTKLLCD 

       370        380        390        400        410        420 
GWWAKSQHIN YFGDWLISLS WCLATWFQTP LTYYYSLYFA TLLLHRQQRD EHKCRLKYGE 

       430 
NWEEYERKVP YKIIPYVY 

P32462 in FASTA format

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View entry in raw text format (no links)
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