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UniProtKB/Swiss-Prot entry P32746


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PYRD_ARATH
Primary accession number P32746
Secondary accession number Q9FMX1
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on December 6, 2002 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 77)
Name and origin of the protein
Protein name Dihydroorotate dehydrogenase, mitochondrial [Precursor]
Synonyms DHOdehase
EC 1.3.3.1
Dihydroorotate oxidase
Gene name
Name: PYRD
OrderedLocusNames: At5g23300
ORFNames: MKD15.16
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=1303803 [NCBI, ExPASy, EBI, Israel, Japan]
Minet M., Dufour M.E., Lacroute F.;
"Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs.";
Plant J. 2:417-422(1992).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/S0014-5793(02)03425-7; PubMed=12372626 [NCBI, ExPASy, EBI, Israel, Japan]
Ullrich A., Knecht W., Piskur J., Loeffler M.;
"Plant dihydroorotate dehydrogenase differs significantly in substrate specificity and inhibition from the animal enzymes.";
FEBS Lett. 529:346-350(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/4.6.401; PubMed=9501997 [NCBI, ExPASy, EBI, Israel, Japan]
Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones.";
DNA Res. 4:401-414(1997).
[4]
IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1105/tpc.016055; PubMed=14671022 [NCBI, ExPASy, EBI, Israel, Japan]
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.;
"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins.";
Plant Cell 16:241-256(2004).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X62909; CAA44695.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF454729; AAN64025.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB007648; BAB11185.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S23762; S23762.
RefSeq NP_568428.1; -.
UniGene At.8807
3D structure databases
HSSP Q02127; 1D3G. [HSSP ENTRY / PDB]
ModBase P32746.
Organism-specific databases
TAIR At5g23300; -.
Gene expression databases
ArrayExpress P32746; -.
GermOnline AT5G23300; Arabidopsis thaliana.
Family and domain databases
InterPro IPR013785; Aldolase_TIM.
IPR005719; DHO_DHase_2.
IPR001295; Dihydroorotate_DHase_core.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.70; Aldolase_TIM; 1.
Pfam PF01180; DHO_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01036; pyrD_sub2; 1.
PROSITE PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.
BLOCKS P32746.
Genome annotation databases
GeneID 832394; -.
GenomeReviews BA000015_GR; AT5G23300.
KEGG ath:AT5G23300; -.
Other
ProtoNet P32746.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Pyrimidine biosynthesis; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    62  62     Mitochondrion (Potential). 
CHAIN   63   460  398     Dihydroorotate dehydrogenase, mitochondrial. PRO_0000029889
ACT_SITE   277   277        Nucleophile (By similarity). 
Sequence information
Length: 460 AA [This is the length of the unprocessed precursor] Molecular weight: 48547 Da [This is the MW of the unprocessed precursor] CRC64: E8DF1C6CCEA44FFD [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAGRAATSSA KWAREFLFRR VSSNPLGATR NCSSVPGASS APKVPHFSKR GRILTGATIG 

        70         80         90        100        110        120 
LAIAGGAYVS TADEATFCGW LFNATKVVNP FFALLDAEFA HKLAVSAAAR GWVPREKRPD 

       130        140        150        160        170        180 
PAILGLEVWG RKFSNPIGLA AGFDKNAEAT EGLLGMGFGF VEVGSVTPVP QEGNPKPRIF 

       190        200        210        220        230        240 
RLSQEGAIIN RCGFNSEGIV VVAKRLGAQH GKRMLAETSA TSSSPSDDVK PGGKSGPGIL 

       250        260        270        280        290        300 
GVNLGKNKTS EDAAADYVQG VHNLSQYADY LVINVSSPNT AGLRMLQGRK QLKDLVKKVQ 

       310        320        330        340        350        360 
AARDEMQWGD EGPPPLLVKI APDLSRGELE DIAAVALALH LDGLIISNTT VSRPDAVSNN 

       370        380        390        400        410        420 
PVATETGGLS GKPLFALSTN MLRDMYTLTR GKIPLIGCGG VSSGEDAYKK IRAGATLVQL 

       430        440        450        460 
YTGFAYGGPA LIPQIKEELV KCLERDGFKS IHEAIGADHR 

P32746 in FASTA format

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