ID   PYRD_ARATH              Reviewed;         460 AA.
AC   P32746; Q9FMX1;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2002, sequence version 2.
DT   25-NOV-2008, entry version 79.
DE   RecName: Full=Dihydroorotate dehydrogenase, mitochondrial;
DE            Short=DHOdehase;
DE            EC=1.3.3.1;
DE   AltName: Full=Dihydroorotate oxidase;
DE   Flags: Precursor;
GN   Name=PYRD; OrderedLocusNames=At5g23300; ORFNames=MKD15.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=93272056; PubMed=1303803;
RA   Minet M., Dufour M.E., Lacroute F.;
RT   "Complementation of Saccharomyces cerevisiae auxotrophic mutants by
RT   Arabidopsis thaliana cDNAs.";
RL   Plant J. 2:417-422(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=22260024; PubMed=12372626; DOI=10.1016/S0014-5793(02)03425-7;
RA   Ullrich A., Knecht W., Piskur J., Loeffler M.;
RT   "Plant dihydroorotate dehydrogenase differs significantly in substrate
RT   specificity and inhibition from the animal enzymes.";
RL   FEBS Lett. 529:346-350(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=98162728; PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA   Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. III.
RT   Sequence features of the regions of 1,191,918 bp covered by seventeen
RT   physically assigned P1 clones.";
RL   DNA Res. 4:401-414(1997).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14671022; DOI=10.1105/tpc.016055;
RA   Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA   Millar A.H.;
RT   "Experimental analysis of the Arabidopsis mitochondrial proteome
RT   highlights signaling and regulatory components, provides assessment of
RT   targeting prediction programs, and indicates plant-specific
RT   mitochondrial proteins.";
RL   Plant Cell 16:241-256(2004).
CC   -!- CATALYTIC ACTIVITY: (S)-dihydroorotate + O(2) = orotate +
CC       H(2)O(2).
CC   -!- COFACTOR: Binds 1 FAD per subunit.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo
CC       pathway; UMP from HCO(3)(-): step 4/6.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane.
CC   -!- SIMILARITY: Belongs to the dihydroorotate dehydrogenase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB11185.1; Type=Erroneous gene model prediction;
CC       Sequence=CAA44695.1; Type=Frameshift; Positions=431;
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DR   EMBL; X62909; CAA44695.1; ALT_FRAME; mRNA.
DR   EMBL; AF454729; AAN64025.1; -; mRNA.
DR   EMBL; AB007648; BAB11185.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S23762; S23762.
DR   RefSeq; NP_568428.1; -.
DR   UniGene; At.8807; -.
DR   HSSP; Q02127; 1D3G.
DR   GeneID; 832394; -.
DR   GenomeReviews; BA000015_GR; AT5G23300.
DR   KEGG; ath:AT5G23300; -.
DR   TAIR; At5g23300; -.
DR   ArrayExpress; P32746; -.
DR   GermOnline; AT5G23300; Arabidopsis thaliana.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004158; F:dihydroorotate oxidase activity; IEA:InterPro.
DR   GO; GO:0006207; P:'de novo' pyrimidine base biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005719; DHO_DHase_2.
DR   InterPro; IPR001295; Dihydroorotate_DHase_core.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF01180; DHO_dh; 1.
DR   TIGRFAMs; TIGR01036; pyrD_sub2; 1.
DR   PROSITE; PS00911; DHODEHASE_1; 1.
DR   PROSITE; PS00912; DHODEHASE_2; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; FAD; Flavoprotein; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; Oxidoreductase; Pyrimidine biosynthesis;
KW   Transit peptide.
FT   TRANSIT       1     62       Mitochondrion (Potential).
FT   CHAIN        63    460       Dihydroorotate dehydrogenase,
FT                                mitochondrial.
FT                                /FTId=PRO_0000029889.
FT   ACT_SITE    277    277       Nucleophile (By similarity).
SQ   SEQUENCE   460 AA;  48547 MW;  E8DF1C6CCEA44FFD CRC64;
     MAGRAATSSA KWAREFLFRR VSSNPLGATR NCSSVPGASS APKVPHFSKR GRILTGATIG
     LAIAGGAYVS TADEATFCGW LFNATKVVNP FFALLDAEFA HKLAVSAAAR GWVPREKRPD
     PAILGLEVWG RKFSNPIGLA AGFDKNAEAT EGLLGMGFGF VEVGSVTPVP QEGNPKPRIF
     RLSQEGAIIN RCGFNSEGIV VVAKRLGAQH GKRMLAETSA TSSSPSDDVK PGGKSGPGIL
     GVNLGKNKTS EDAAADYVQG VHNLSQYADY LVINVSSPNT AGLRMLQGRK QLKDLVKKVQ
     AARDEMQWGD EGPPPLLVKI APDLSRGELE DIAAVALALH LDGLIISNTT VSRPDAVSNN
     PVATETGGLS GKPLFALSTN MLRDMYTLTR GKIPLIGCGG VSSGEDAYKK IRAGATLVQL
     YTGFAYGGPA LIPQIKEELV KCLERDGFKS IHEAIGADHR
//