ID   G3P2_CAEBR              Reviewed;         341 AA.
AC   P32809; A8XJE1;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-NOV-2008, entry version 58.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 2;
DE            Short=GAPDH-2;
DE            EC=1.2.1.12;
GN   Name=gpd-2; Synonyms=gpd-3.2; ORFNames=CBG14137;
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93077036; PubMed=1446820; DOI=10.1016/0378-1119(92)90126-A;
RA   Lee Y.H., Huang X.Y., Hirsh D., Fox G.E., Hecht R.M.;
RT   "Conservation of gene organization and trans-splicing in the
RT   glyceraldehyde-3-phosphate dehydrogenase-encoding genes of
RT   Caenorhabditis briggsae.";
RL   Gene 121:227-235(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A.,
RA   D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E.,
RA   Griffiths-Jones S., Harris T.W., Hillier L.W., Kamath R.,
RA   Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P.,
RA   Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M.,
RA   Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M.,
RA   Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for
RT   comparative genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; M86669; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CAAC02000521; CAP32766.1; -; Genomic_DNA.
DR   PIR; JH0769; JH0769.
DR   HSSP; P56649; 1DSS.
DR   SMR; P32809; 3-340.
DR   Ensembl; ENSCBRG00000002093; Caenorhabditis briggsae.
DR   WormBase; WBGene00000332; Cbr-gpd-3.2.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DHase.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DHase_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    341       Glyceraldehyde-3-phosphate dehydrogenase
FT                                2.
FT                                /FTId=PRO_0000145508.
FT   NP_BIND      13     14       NAD (By similarity).
FT   REGION      157    159       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      217    218       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    158    158       Nucleophile (By similarity).
FT   BINDING      35     35       NAD (By similarity).
FT   BINDING      85     85       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     188    188       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     240    240       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     322    322       NAD (By similarity).
FT   SITE        185    185       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   341 AA;  36509 MW;  C93984A33A4A253E CRC64;
     MSKPTVGING FGRIGRLVLR AAVEKDSVNV VAVNDPFISI DYMVYLFQYD STHGRFKGTV
     KHEGDYLIVA NEGKSQHKIK VYNSKDPAEI QWGAAGADYV VESTGVFTTI EKANAHLKGG
     AKKVIISAPS ADAPMFVVGV NHEKYDHAND HIISNASCTT NCLAPLAKVI NDNFGIIEGL
     MTTVHAVTAT QKTVDGPSGK LWRDGRGAGQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR
     VPTPDVSVVD LTARLEKPAS LDDIKRVIKA AAEGPLKGVL AYTEDQVVST DFVSDTHSSI
     FDAGASIILN PNFVKLISWY DNEFGYSNRV VDLISYIATK A
//